...guide you through the basics of CombiGEM-CRISPR experiments. CombiGEM-CRISPR: The marriage of two simple...gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12...allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal ... complex regulation in biological systems. The CombiGEM platform provides a means to create barcoded gRNA..., 2014; Wong et al., 2015; Wong et al., 2016). CombiGEM uses iterative one-pot reactions with straightforward...quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can accommodate any genetic...address the users’ specific research questions. CombiGEM has been successfully applied to functionally ...

...can feel ineffective or unimportant. With so many big contributors to the sustainability problem, how can...reusable water bottle instead of buying yourself a big stack of plastic cups. By initiating little changes...lot of energy, so even one less run a week makes a big difference. Clean out freezers so you can make room...behavior can lead to cultural shifts that can have bigger impacts. For example, if you joined an office where...

...Addgene’s homepage recently, you probably noticed some big changes to our site (if you haven’t, go check it ...gets a make-over You may also have noticed another big update on the homepage. Blugene, our trusted friend...promote our values of community and sharing.  One big feature upgrade? Blugene now has a full expression...

...take a week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute...depositor fun facts for a quick way to grasp the big impacts depositors have on science globally. *All...

...policy, there is often discussion of “big P” and “little p” policy.  “Big P” policy is what you might typically...can also happen at the state and local level. The big secret about science policy that I didn’t learn until...the government itself. You can work to influence Big P policy from a post in non-governmental organizations... scientific areas. Whether you’re interested in “big P” or “little P” policy, the possibilities are endless...

...Why is choosing the right lab such a big deal? It’s actually something you CAN choose and it will make...gratefully moved on. My second rotation was in a big lab with a famous PI who I was only likely to see...the atmosphere that will allow you to thrive. How big is the group? Has it grown over time? If not, why...asking for and getting help, size might not be a big factor for you. Know who's boss You sign on to a...about this. The training you get now will have a big impact on everything you do in the future. Questions...

...person and with a group who all strive for a common big-picture goal. It is worth doing some some serious...understand your own style before making this choice.  How big is the lab? While larger labs tend to be more "chaotic...the advisor, you should choose accordingly. In a big lab, the advisor might not be around much and your...

...control. And, like all controls, missing out is a big mistake. Chromatograms come to the rescue for analyzing...sequencing facility requires you to perform your own Big Dye PCR amplification reaction (as opposed to using... the sequence may suggest. In fact this is so ambiguous that the DNA sequencing reaction should be repeated...

...Work on a big project that relies on a division of labor Serve on a committee and do something big like plan...

...editing's PE2 enzyme do not. So how do you fit a big gene into a tiny vector like AAV? By breaking the... lab decided to solve the issue of “tiny vector, big transgene” by just straight up packaging an oversized...combination of serotype and tissue/cell type could have a big impact on the rate of transgene expression with an...Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction: Fragmented Genome Reassembly of...

...incredibly helpful to me to know that you can make big changes in your career path and still find comfort...wondered what my coworkers value and if that is a big part of why they work at Addgene. I posed this question... nonprofit finance, and while I've done that, my big takeaway has been the impact I can have on an organization...Overall, the Meet an Addgenie project was one of the biggest takeaways from my co-op. It has been so insightful...

...circulating tumor cells (CTCs) is going to have a big impact on our understanding and treatment of cancer... may well be of use to others to Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase...

...know more. This could be by outright stating the big-picture problem, by asking your audience a simple...necessary and what can be left out, which can be a big challenge!  The resolution Throughout your conflict...

...laboratory sets so we had to purchase the big LEGO classic box, the Big Bang Theory set, and then use our imaginations...

...Table 1: Taking it a Step Further - Big Company v. Small Company Big (e.g. Pharma) Small (e.g. Biotech...

...Boyden about this latest optogenetics advance, the big questions that motivate his interest in neuroengineering...optogenetics research Addgene: What are some of the big problems that optogenetic tools can help us to address... that require less power. Those are two of the biggest ones. And as a third example, it would be helpful...

...Biochemical Research Methods. We also pulled the big interdisciplinary journals: Nature, Science, PLOS...caveat, it is still suggestive that we see such a big difference in citations after controlling for journal...

...Good science isn’t the only facet of a project this big. Maintaining our excellent service and accuracy involves...to do this well ... because it did. So finally, a big thank you to the Addgenies who went the extra mile...recognition, further fulfilling our mission for an even bigger impact. In early 2016 we surveyed hundreds of scientists...

... from someone you trust. A good coach can make a big difference, if you can find one. Create your own ...ask someone to be your mentor, it may sound like a big responsibility or commitment. Start by asking them... with ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around...

...stock of antibiotic. Many small colonies surrounding big colonies. You probably left your plate for too long...has been used. Don’t worry too much, just pick the big colony and you are safe! Colony screening Pick a...