We narrowed to 42 results for: /1000
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TypeBlog PostPublishedJuly 14, 2017, 2:30 p.m.... Commercial Plasmid Prep Kit Amount 1000 Lanes 1000 Lanes 1000 Lanes Cost $200-$500 <$10 $20-30 ...convenient, commercial ladders cost between $200-$500 for 1000 lanes worth of ladder. The Penn State ladders are...plasmid prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder. Using...
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Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost and the Dark Lord rules ...Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m.... but a select few are particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell™ reduces every... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your downstream analysis... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....two-photon imaging with newer lasers in the range of 1000–1080 nm. These industrial fiber lasers are becoming... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed PMID: 17072308. 4. Dieck, Tom, et al. "... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....and very cool 8,768,560 hours – or almost exactly 1000 calendar years! *Dammit, man, I’m a scientist... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....particles. Studies have found that values can be 10-1000 fold higher than for functional assays depending... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....Beroukhim and Boehm labs pulled data from more than 1000 CRISPR screens in DepMap to see if they could identify... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....coefficient and quantum yield of the protein, divided by 1000. In many cases the brightness is compared to that... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m.... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....includes SpCas9, and shortern orthologs approximately 1000 amino acids in size. From the pool of shorter orthologs... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....proteins are quite large — comprising approximately 1000–1400 amino acids — which poses a challenge for efficient... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....of template should have homology arms between 500-1000 bps. HDR efficiency with circular plasmid templates... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....design 5 guide RNAs (gRNAs) targeting each of the 1000 genes you’d like to investigate in your next CRISPR... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m.... E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID: 25822415. Find plasmids from this... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....Low 572/ 610 FP595 Tetramer Green Dark/ Red 70 - 1000 Medium 590/ 600 Choosing a photoactivatable... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....fibroblasts to myoblasts. Cell, 1987. 51(6): p. 987-1000. PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic...