...pAAV_hSyn-PdCO-mScarlet-WPRE AAV5 Optogenetics Ofer Yizhar New viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE AAV1, AAV5...Boyden New serotype pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE AAV1 Optogenetics Karl Deisseroth New serotype ...Karl Deisseroth New serotype AAV-CamKIIa-jGCaMP8s-WPRE AAV5 Biosensors Loren Looger New serotype pAAV-CAG-FLEx-iSeroSnFR...Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2-WPRE AAV1, AAV5 Biosensors GENIE Project New viral ...multiple serotypes pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE AAV1, AAV5 Biosensors GENIE Project New viral ...with multiple serotypes pGP-AAV-syn-iGABASnFR2-WPRE AAV1, AAV5 Biosensors GENIE Project New viral ...multiple serotypes pGP-AAV-syn-iGABASnFR2(no bind)-WPRE AAV1, AAV5 Biosensors GENIE Project New viral ...

...below: pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-...pGP-AAV-syn-jGCaMP7c variant 1513-WPRE (105321-AAV1) pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (105322-AAV1) GABA...Sensors pGP-AAV-syn-jGCaMP7b-WPRE (104489-AAVrg) pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics pAAV-hSyn...directs broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous...pAAV-EF1a-fDIO-Cre (121675-AAV9, 121675-AAVrg)  pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1...

...AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor Looger...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-WPRE  AAV9 Biosensor Looger...pAAV-hSyn-JEDI-2P-Kv-WPRE AAV9 Biosensor St-Pierre New viral vector pAAV-hSyn-JEDI-2P-WPRE AAV9 Biosensor ...Chemogenetics Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral ...viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV...

...AAVrg pGP-AAV-syn-jGCaMP7b-WPRE 104493 AAVrg pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s...124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1...Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO mCherry 99130...Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene...

...service with multiple serotypes pAAV_hSyn-PdCO-EGFP-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service...multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service...Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE PHP.eB Biosensors GENIE Project New serotype pAAV-hSyn-GRAB_OT1.0...Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service...Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral service...multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral service...

...-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262  AAV1 pAAV-hSynapsin1... pAAV-EF1a-Flpo 87306  AAV1 AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics...105322  AAV1  pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics...124603  AAV9  pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40  Additional resources on the Addgene blog... Plasmid Serotype Name 105530  AAV1 pAAV.CMV.PI.EGFP.WPRE.bGH 37825  AAV1 pAAV-CAG-GFP 59462  AAV2...

...Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.F102G...soon”). The 7b variant (pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of ...Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity...105321  AAV1  pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between peak fluorescence...  AAV1  pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE7c exhibits high contrast between peak fluorescence...

...system.  WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a...helps increase the expression of your transgene. The WPRE is located at the end of the AAV genome, just upstream... upstream of the final ITR and downstream of the WPRE. TL;DR? Watch our AAV Transfer Plasmid video! ...

...preps here: pAAV-EF1a-DIO-JEDI-2P-WPRE and pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE   Liu Z, et al. Sustained deep-...AAV1) pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...(AAV1) pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (AAV1) pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 (...pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)   There are hundreds of other biosensors in Addgene’s catalog — from...

...RNA target site for packaging by Nucleocapsid. WPRE Woodchuck hepatitis virus post‐transcriptional...

...targeting specific viral components such as LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself...

...-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn GCaMP7s, Cre-dependent...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG GCaMP7s...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f...-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE Syn GCaMP8s...pGP-AAV-syn-jGCaMP8m-WPRE Syn GCaMP8m Calcium sensor GENIE 162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn GCaMP8f Calcium...pGP-AAV-syn-FLEX-jGCaMP8s-WPRE Syn GCaMP8s, Cre-dependent Calcium sensor GENIE 162378 pGP-AAV-syn-FLEX-jGCaMP8m-WPRE Syn GCaMP8m...pGP-AAV-syn-FLEX-jGCaMP8f-WPRE Syn GCaMP8f, Cre-dependent Calcium sensor GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG GCaMP8s...

...pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-jGCaMP8f-WPRE Syn jGCaMP8f...AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f...pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE Syn ...AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 9, rg* Looger 176752 AAV-CamKIIa-jGCaMP8s-WPRE CamKIIa ...-jGCaMP8s-WPRE Dlx jGCaMP8s none Constitutive 1, 9, rg* Looger 176758 AAV-EF1a-jGCaMP8s-WPRE EF1a jGCaMP8s...pGP-AAV-syn-FLEX-jGCaMP8m-WPRE Syn jGCaMP8m none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m...AAV-Syn-H2B-jGCaMP8m-WPRE Syn jGCaMP8m none Constitutive 1, 9, rg* Looger 176751 AAV-CamKIIa-jGCaMP8m-WPRE CamKIIa ...

...-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use weaker poly(A) signals...

...tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 Eric...pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-...AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH James M. Wilson AV...AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-9-PV0101 105530-AAV9 pAAV.CMV.PI.EGFP.WPRE.bGH James M. Wilson AV...-EYFP-WPRE-HGHpA Karl Deisseroth AV-1-20938M 100054-AAV1 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Karl ...-EYFP-WPRE-HGHpA Karl Deisseroth AV-5-20938M 100054-AAV5 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Karl ...-EYFP-WPRE-HGHpA Karl Deisseroth AV-9-20938M 100054-AAV9 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Karl ...

...mScarlet-WPRE CaMKII eOPN3 mScarlet Constitutive 1, 5 Yizhar 125713 AAV-hSyn1-SIO-eOPN3-mScarlet-WPRE Syn ...pAAV_hSyn-PdCO-mScarlet-WPRE Syn PdCO mScarlet Constitutive 5 Yizhar 198511 pAAV_hSyn-DIO-PdCO-mScarlet-WPRE Syn PdCO...-EGFP-WPRE Syn PdCO EGFP Constitutive 1, 5 Yizhar 198516 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE EF1a ...20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre dependent 1, 5... 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP Cre dependent 1, 5, 9...Adesnik 124603 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 EF1a ChrimsonR (soma-targeted) mRuby2 Cre dependent...Harvey 130991 pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) ...

...the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available in AAV5 and AAVrg...

...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG...-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn Calcium sensor...2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Control Feng 163505 CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA minBetaGlobin...pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor RGECO Kim , GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn Calcium... 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent ChR2 Deisseroth...Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP...

...expression. Stabilizing the gvpA transcript with WPRE reduced the number of copies of gvpA needed to increase...