...eukaryotic genomes.  Components of a transposon system Transposon: The transposon is the DNA sequence that moves... piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was ...transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve learned...also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons are commonly...Sleeping Beauty. Minimal Tol2 or miniTol2 is a truncated version of the original Tol2 and has ~3-fold increase...discovered transposons due to changes in coloration of corn kernels. Aside from corn, transposons are in ...2010).  So why do transposons matter? The outcome really depends on where a transposon lands. For example...

.... One common and robust strategy uses a transposon called Tol2 to randomly integrate foreign DNA into ...relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish genome...Mosimann. "Contemporary zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments...

...neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit generated by the Chi-Bin...to get all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous...nervous system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists... Dyn. 2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact...comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different variants ...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at ...

...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination,... Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders...