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Showing: 1 - 7 of 7 results
  1. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...variant of oil palm." Nature 525.7570 (2015): 533-537. PubMed PMID: 26352475. 2. Bygren, Lars O., et al....sotires like the potential devastation of the palm oil industry through epigenetic effects on the Cover ...Central PMCID: PMC3929550. 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and ...that compacts cellular DNA into chromosomes and reduces the risk of dangerous DNA damage. If the way DNA...studying disease Epigenetic changes have been discovered to play a large role in a variety of diseases...passed on a trait to their granddaughters that reduced their lifespan. Because these dramatic conditions...developing, an epigenetic change is thought to have occurred that was passed down to the grandchildren; in ...
  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Cohen Redox/ROS Red fluorescent biosensor for glutathione redox changes Red fluorescent redox-sensitive...4153. Masamitsu Iino Calcium Red mitochondrial CEPIA calcium sensors Red fluorescent CEPIA indicators ...Calcium Red fluorescent calcium sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent... cAMP (cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based...Kitaguchi Glucose Red fluorescent glucose indicators for living cells (Glifons) Development of red genetically...Julius Zhu Dopamine Red and green GRAB_DA3 dopamine sensors Improved green and red GRAB sensors for monitoring...David Raible pH Red or Orange pH biosensors (pHuji and pHoran4) pHuji, a pH-sensitive red fluorescent protein...
  3. CRISPR Guide

    Type
    Collection
    ...Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., Nureki, O., & Zhang...., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., Oura, S., Holmes,...350. PMID: 25533786 Cas9 alternatives Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S....28976959 Cox, D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang...792. PMID: 28581492 Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung....2022.100013 Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler...E. M., Winblad, N., Choudhury, S. R., Abudayyeh, O. O., Gootenberg, J. S., Wu, W. Y., Scott, D. A., Severinov...
  4. CRISPR Guide

    Type
    Guide
    ...Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., Nureki, O., & Zhang...., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., Oura, S., Holmes,...350. PMID: 25533786 Cas9 alternatives Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S....28976959 Cox, D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang...792. PMID: 28581492 Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung....2022.100013 Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler...E. M., Winblad, N., Choudhury, S. R., Abudayyeh, O. O., Gootenberg, J. S., Wu, W. Y., Scott, D. A., Severinov...
  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... 35 μL ddH 2 O Incubate for 4 minutes at 95°C in a PCR machine or in a beaker of boiling water. If using...likely to shear the envelope of your virus. o. Virus may be stored at 4°C for a few days, but should be frozen...miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI add ddH 2 O to bring to 50 μL final volume Incubate at 37°C for...Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate with EcoRI. Mix: 30 μL pLKO.1 TRC-cloning...μL 10x NEB buffer for EcoRI 1 μL EcoRI 14 μL ddH 2 O Incubate at 37°C for 2 hours. Run digested DNA on ...Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA concentration. C.4 Ligating and Transforming...DNA ligase buffer 1 μL NEB T4 DNA ligase add ddH 2 O to bring to 20 μL final volume Incubate at 16°C for...
  6. Pouring LB Agar Plates

    Type
    Protocol
    ...chloride 12.0 g agar-agar 1 L Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm...indicated, the antibiotic powder can be dissolved in dH 2 O. *Carbenicillin can be used in place of ampicillin...with an absorbent material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your... with resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance...LB-agar powder. Transfer the LB-agar powder you’ve measured out into an appropriately sized bottle for autoclaving... is necessary to prevent your molten agar from boiling over in the autoclave. Transfer the sterile water...the opening of the bottle with its cap or aluminum foil (but do not make an air-tight seal!) and tape the...
  7. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has pre-mixed LB agar ...the suggested amount, instead of the other dry ingredients above. Media without growth (top) and with growth...cover the entire top of the bottle with aluminum foil. Autoclave and allow to cool to room temperature... Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate bacterial...
Showing: 1 - 7 of 7 results