...from Transposagen What is the piggyBac™ transposon? The piggyBac™ transposon was originally identified in... other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac™ transposase...plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase...genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful...with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon system...using the piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor...ITRs) found on the piggyBac™ donor for its activity. In summary, the piggyBac™ transposon is a powerful technology...

...eukaryotic genomes.  Components of a transposon system Transposon: The transposon is the DNA sequence that moves...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...about the Sleeping Beauty DNA transposon Learn about the piggyBac DNA transposon ...also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons are commonly...discovered transposons due to changes in coloration of corn kernels. Aside from corn, transposons are in ...2010).  So why do transposons matter? The outcome really depends on where a transposon lands. For example...components of transposons, how they are classified, and how they are used in the lab. What is a transposon? DNA...

...lentiviral expression of OSKM reprogramming factors: Mouse Expression Vectors Human Expression Vectors... residual expression piggyBac Excisible Medium Medium Generates transgene-free and vector-free cells...for therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element that...harmful viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic...so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming...reprogramming. Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells... retroviral vectors. This makes it possible to use a polycistronic cassette that expresses multiple reprogramming...

...fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs...the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells Lindy...CRISPRa depended on continued expression of dCas9 and gRNAs whose expression varies depending on transgene...demethylation Optogenetic repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic...optogenetic toolkit that controls repression of gene expression in Saccharomyces cerevisiae. The system consists...the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the NLS is inaccessible and the...of CRISPRi in Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo...

... AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression) Mammalian Plasmid...modular vector assembly process designed for a quick, plug-and-play approach to cloning CRISPR vectors (McGee...for each module, generate the vector file, and quickly clone their vector of interest. And it truly is ...destination vectors for various delivery methods, along with compatible inserts. All destination vectors contain...heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right for your...Fragmid destination vectors currently available. Table 1: Fragmid destination vectors Organism Delivery...designing a CRISPR vector the Fragmid way!    Figure 1: Selecting your vector type on the Fragmid...

...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at ...to comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different variants...low off-target activity; mammalian expression vector Rees H, et al. Nature Communications. 2017. PubMed...and ribozyme systems for multiplex gRNA expression. Expression of toolkit components can be controlled...gene editing. The bacterial and mammalian expression vectors for these CRISPR inhibitors are listed in...Species Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9...TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites or gateway compatible...

...Yamanaka piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse Oct4, Sox2, Klf4, and c-Myc...Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 ...epigenetic and gene expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible...

...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...

...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...