...-BY license.   Find MTRIA sensors here!   Ino D, et al. A fluorescent sensor for real-time measurement...interest, they create sensors for FGFR1, EGFR and ErbB2. And orthogonal sensors with different ITAM/SH2... new viral preps of the dopamine sensor dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs...hottest fluorescent biosensors new to Addgene this year. Feel the love with MTRIA sensors One of the latest... class of sensors for neuropeptides and neurotransmitters. Motivated to develop a biosensor for oxytocin...engineering improved sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting... potassium sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a ...

...for a sensor for a different analyte, a sensor for gene-specific activity, or for popular biosensors in ...Calcium GCaMP-X genetic encoded calcium sensor Improved calcium sensor GCaMP-X overcomes the calcium channel...FRET-based zinc sensor for imaging in live cells (CALWY) Genetically encoded FRET sensors to monitor intracellular...+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic...Genetically-Encoded Sensors. Anal Chem. 2017 Jan 3;89(1):711-719. Amy Palmer Zinc Cyan-Yellow Zinc FRET sensors ZapCY1...using optical sensors. Biochem J. 2010 Dec 1. Wolf Frommer ATP yAT1.03 FRET-based ATP sensor for yeast An...fluorescent sensor. PLoS One. 2017 Nov 9;12(11):e0187481. Mathew Tantama ATP QUEEN ATP sensors for live-...

...2019, the first generation of norepinephrine sensors was developed, named GRABNE, which helped researchers...from Kagiampaki et al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG...GRABNE is blocked by alpha2-AR antagonist, nLight sensors are not. Instead, they are blocked by alpha1-AR...advantages of this next generation of norepinephrine sensors is their speed. nLightG is activated eight times...selectivity for norepinephrine over dopamine. All three sensors, however, did display some reaction to high concentrations...Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine...brain but have been difficult to study without biosensors to directly visualize their activity. In 2019...

...in the 3’ UTR by four miRNA target sites and thereby serves as a sensor of miRNA presence and activity...field further with the creation of the largest miRNA sensor collection reported to date, containing all ...PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated gene regulation...Listen to the miRNA reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized... a subset of active miRNA’s unique to HEK293FT and HeLa cell lines. When the miRNA activity profile for...Despite several years of research, the mechanisms of miRNA regulation have yet to be fully elucidated. Ron ...all 620 sequences of probable mature human miRNAs. Weiss’s collection consists of a library of reporter...

...research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT ....2021.01.003. PMID: 33714753. Voltron2 voltage sensors By Brian O’Neill New viral vector preps expressing...neurons in vivo. Because they are negative-going sensors (with fluorescent signal at baseline voltage), ... specificity of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence transgene...AAV vectors, either alone or in combination with miR124T targeting sequences, provides a simple yet valuable...

...processes, biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created by...destabilized Firefly luciferase mutants : A set of three sensors that are increasingly structurally destabilized...Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore...permuted Venus fluorophore. Learn more about pHlash sensor in our 28 Hot Plasmid Technologies from 2015 blog... for assays of transcriptional regulation and biosensors. Plasmid... reporter gene in vitro and in vivo to use in biosensors. The most common types of luciferase found in...during T urnover) : Single-molecule mRNA turnover biosensor that utilizes Renilla luciferase and a Halo tag...

...-6. doi: 10.1038/nmeth.3147. Bioluminescent pH sensor Understanding intracellular pH regulation is important...events. To overcome the issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters... biology tools by engineering light-switchable sensors developed from bacterial two-component signal transduction...transcription activation or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent... and 4) optimizing expression of both the light sensor and the response regulator. These changes reduced...expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression, or...or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene deletion, ...

... optogenetics. For information on sensors, check out our biosensors collection. Microbial Opsins Opsins...proteins; e.g., microbial opsins and optical switches Sensors are genetically-encoded reporters of molecular ...Light-oxygen-voltage-sensing (LOV) domains are protein sensors used by many plants, microalgae, fungi and bacteria... Neurosci. 8(9):1263-8. PMID 16116447 Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sørensen ...

...engineered ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category...effectively deploying them in the lab somewhat mirrors the complexity of understanding their wild counterparts...large percentage of viral vector preps contain biosensors, optogenetic tools, and chemogenetic tools: protein... most commonly requested (Figure 3). Over 400 biosensors were distributed in 2023 alone. You’ll also find...in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first developed...

...Knockout Human Li 3rd ~7 11,364 Dow Lab Base Editing Sensor Libraries 179217-179219 Base Editing Human, Mouse...113345 Activation Human Wright 3rd 7-14 58,570 Human miRNA CRISPR Knockout Library 112200 Knockout Human Lin...

...transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting...Gene regulatory elements [promoters and enhancers, miRNA target sites, and recombination target sequences...expression to your cell type of interest, while miRNA target sites can be used to prevent expression in... chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up precious cargo...immune response against the capsids or transgenes. Sensory neurons and liver appear especially susceptible...PNS, including ENS (broad): somatic and visceral sensory ganglia, type II spiral ganglion neurons Mice...Chaudhari, N. (2022). Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S. eNeuro,9(3), ...

.... FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova...Enhanced miRFPs are monomeric and bright Recent series of enhanced monomeric NIR FPs named miRFPs (monomeric...Therefore, we recommend using miRFPs and their enhanced versions named emiRFPs in your applications.  There...set of spectrally distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome...advantages are their small size (17 kDa for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility... miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 miRFP709 https...

...Vladislav Verkhusha iRFP GAF domains from miRFP709 and miRFP670, pair with common PAS domain. Requires presence...blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors Collection Split ...near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova et al. Nat...

...Hereditary sensory neuropathy type IE Tyler Jacks 12166 pSicoR Dnmt1 DNMT1 GFP CMV Hereditary sensory neuropathy...Hereditary sensory neuropathy type IE Bert Vogelstein 16649 DNMT1 KO Neo DNMT1 T7 Hereditary sensory neuropathy...Hereditary sensory neuropathy type IE George Daley 36383 pSMP-DNMT1_2 DNMT1 Hereditary sensory neuropathy...Clifford Brangwynne 122441 pHR-FUSN-miRFP670-Cry2WT FUS Cry2WT, miRFP670 SFFV ALS Clifford Brangwynne 122668...Nico Dantuma 24952 LZRS-DNMT1 DNMT1 Hereditary sensory neuropathy type IE Paul Khavari 25044 pDEST53-LRRK2...Mark Cookson 25165 DNMT1 DNMT1 His T7 Hereditary sensory neuropathy type IE Cheryl Arrowsmith 25361 2XMyc-LRRK2...George Daley 36384 pSMP-DNMT1_3 DNMT1 Hereditary sensory neuropathy type IE George Daley 36805 TAL2402 ATM...

...Filaments LifeAct miRFP703 Vladislav Verkhusha 79994 pEB3-miRFP703 Microtubules EB3 miRFP703 Vladislav Verkhusha...GFP Pantelis Tsoulfas 80000 pMito-miRFP703 Mitochondria COX8A miRFP703 Vladislav Verkhusha 36208 pmTurquoise2... mCherry Robert Benezra 80001 pH2B-miRFP703 Chromatin H2B miRFP703 Vladislav Verkhusha 17650 GFP-HP1gamma...Verkhusha 79990 pKeratin-miRFP703 Intermediate Filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha ...Verkhusha 79991 pmiRFP703-Tubulin Microtubules alpha-tubulin miRFP703 Vladislav Verkhusha 79993 pLifeAct-miRFP703...Verkhusha 79996 pVimentin-miRFP703 Intermediate Filaments (vimentin) Vimentin miRFP703 Vladislav Verkhusha ...Vladislav Verkhusha 79999 pZyxin-miRFP703 Focal Adhesions Zyxin miRFP703 Vladislav Verkhusha 55023 mCherry-Cx43...

...Plasmids miRFP670 642 670 12 4.5 Monomer pmiRFP670-N1 - Mammalian Expression pBAD/His-miRFP670 - Bacterial...Expression miRFP703 673 703 8 4.5 Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial...Expression miRFP709 683 709 4 4.5 Monomer pmiRFP709-N1 - Mammalian Expression pBAD/His-miRFP709 - Bacterial...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection...

...Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson...well as Ligand-Independent Functional Changes in Sensory Neurons. The Journal of Neuroscience, 36(42), 10769...