...unfolds under tension. When this happens, BiT and the LgBiT luciferase fragment (expressed separately) assemble...Left: Mechanism of the PILATeS tension sensor. The LgBiT (cyan) fragment of luciferase binds to the BiT Tag... Titin I10 unfolds under tension. Upon binding, LgBiT and BiT Tag catalyze a luminescent reaction with...