...using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral Transfer...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively ...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase...For research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...

...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR...pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n(BB)-2A-GFP (PX461) expresses...pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes both ...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists... be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our...requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the Zhang lab expresses...

...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...choice for a gene transfer vector. Lentiviral vectors Although retroviruses are still in use, considerable...expression vectors to target cells - one containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3)...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ... the genome is more efficient. The first retroviral vectors used were derived from the Moloney Murine...

...pressure on the production of retroviral vectors: Enhancement in vector stability." Biotechnology and ...guide to viral vectors Find all of our viral vector blog posts Download our viral vectors 101 eBook Additional...been actively investigating ways to improve viral vector production. Whether redesigning viral packaging...membrane When packaging adeno-associated viral vectors (AAV) not all serotypes are equal; while some serotypes...increased yields in adenoviral (Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge...endocytosis of a fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference...we believe that this approach can be adapted by vector cores and laboratories alike as an easy and inexpensive...

...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene essential... on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing...components of a virus into different plasmids. For a retroviral system this looks like the genetic cargo in one... researcher.   Figure 1: Separation of retroviral packaging components onto distinct plasmids for...cellular function, this would also be a higher risk vector. While it is unlikely that you, or anyone else ...classification. Lentivirus (a type of retrovirus) and gamma retrovirus require more caution as they are integrating...common types of viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated...

...fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes...will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that ...conduct your custom experiment. Fly vectors The vast majority of fly vectors are based on p-element, random...studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following the...

...Expression Type MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II...II IRES Retroviral pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase... the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to...get started with them. Why Use multicistronic vectors? Detecting cells that are expressing your gene,...expressing your gene of interest at high levels. Unlike vectors that express screenable or selectable markers from...

...murine leukemia virus (MMLV)-derived retroviruses. Retroviral vectors, such as pMXs or pMSCV, infect dividing...safety required for translational use. Viral Vectors Retroviral: Some of the first iPSCs were generated ...corresponding endogenous pluripotency genes. Retroviral vectors are prone to incomplete silencing of reprogramming...cloning capacity (8-10 kb) than other retroviral vectors. This makes it possible to use a polycistronic...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...

... destination vector for gene expression GFP Varies pBABE GFP - Retroviral ...expression pcDNA3 GFP LIC cloning vector - C-terminal GFP for mammalian expression...pPS808 - N-terminal GFP for yeast expression pET GFP - C-terminal GFP for...gene expression pMKO.1 GFP - Retroviral shRNA expression pSico PGK GFP - Conditional... retroviral shRNA expression MSCV-IRES-GFP - Mammalian retroviral gene expression...C elegans expression GFP Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression... bacterial expression vector (see tagged expression vectors from the SGC) pPro18...

...Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid ...MSCV-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...Viral Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro.... Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised...FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common γ-retroviral envelope ...

...48201 CAG-GFP-IRES-CRE Cre and GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG...116879 CAG-Cremyc-2A-GFP GFP and Cre CAG Mammalian Lu 117148 Hiv7CMV-Cremyc-2A-GFP GFP and Cre CMV Lentiviral...CAG Retroviral Gage 49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980 iCre with MCS for inserting... Bacterial Liu 63704 pRetroX GFP T2A Cre GFP and Cre tight TRE Retroviral Foijer 64769 pTW040 Cre fused...neurons Retroviral Luikart 66693 pRubiG-T2A-cre GFP and Cre expression in newborn neurons Retroviral Luikart...iRFP670-EFS:Cre-2A-GFP iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler...13770 pCALNL-GFP Cre dependent GFP expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch...

...lentiviral and retroviral constructs and we will discuss these in a future post on viral vector parts. Promoter... Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...

...Aebischer and Didier Trono Constitutive Retroviral shRNA Retroviral vectors for constitutive shRNA expression...8452 pMKO.1 puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative...Negative control vector for pMKO.1 puro; Contains shRNA against GFP William Hahn 10676 pMKO.1 GFP Derivative...shRNA cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should...the mouse U6 promoter; A CMV-EGFP reporter cassette is included in the vector to monitor expression; Designed...protocol for using the popular cloning vector pLKO.1. Trono Lab Lentivectors Toolbox Tyler Jacks Lab Protocols...Lentiviral Tet-regulated Lentiviral Constitutive Retroviral Resources Additional Resources: Human and mouse...

...option rtTA On Kowarz 16623 pBI-GFP Expression of your gene of interest & GFP from a bidirectional tet-responsive...Harbor Locus. Tet-On 3G On Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for conditional... has rtTA driven by retroviral LTR promoters ; see article for lentiviral vectors for tet-inducible gene...shRNA cloning vector; for evaluation of shRNA efficacy using fluorescence; use with pGFPns-reporter for...rtTA On Campeau 25434 pMA2640 Retroviral; CMV-driven; linked via IRES to EGFP-Blasticidin fusion; pMA2641...Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background Tetracycline...selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway cloning; selection...

...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...interest (analyte), and accumulation of ATP activated GFP expression (receptor), then the transducer could ...plate reader, or any other instrument which measured GFP levels and converted that signal into a readable ...Biosensors Viral Service: Biosensor AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview...For one, many viral platforms, such as AAV or retrovirus, can either permanently integrate and/or express... sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we’ve got you covered...

...lentiviral transfer vector or separate transfer vectors. May contain reporter gene (e.g. GFP) to identify and... create a fusion with a reporter protein such as GFP? All of these outcomes can be most effectively achieved...Components of System Application Mammalian expression vector Promoter driving Cas9 expression can be constitutive...typically used for gRNA. May contain reporter gene (e.g. GFP) to identify and enrich positive cells or a selection...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or...easy Very hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing ...repair by non-homologous end joining (NHEJ) or HDR. EGFP validation of sgRNAs: Can evaluate component delivery...

...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...interest are cloned into a Firefly luciferase reporter vector via Gateway cloning to test for the presence of...pGL4.84(hRlucCP/Puro) RapidResponse(TM) Renilla Vector for investigating regions controlling transcription...Pete Stecha 212933 pGL4.84(hRlucCP/Puro) Renilla Vector for investigating regions controlling transcription...Welm 18760 MSCV IRES Luciferase Firefly (IRES) Retroviral expression of firefly luciferase and gene of ... 18782 MSCV Luciferase PGK-hygro Firefly SV40 Retroviral expression of firefly luciferase Scott Lowe 14080...

...the vector backbone you cloned your insert into and fill out the appropriate boxes with the vector backbone...backbone manufacturer, vector backbone size, total size of your vector + insert, any modifications you ...provide any full or partial plasmid sequences and any vector maps. The more sequence data available, the better... was a part of the insert (when cloned into the vector backbone). If your plasmid has more than one insert...you made to the backbone, and the vector type. Pro Tip! Reference the Addgene ID in the "Backbone Manufacturer...enter "Feng Zhang (Addgene plasmid #52961)".   The vector type should describe how your plasmid is intended...in E. coli, then this is a Bacterial Expression vector. If your plasmid can be classified in multiple ...

...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-...coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...

...protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene 63591...protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene 64325...interest 85586 gRNA Cloning Vector Bbs I ver. 2 An empty sgRNA expression vector. sgRNA sequence can be cloned...associated with an IFNgamma-responsive promoter by a retroviral expression system for enChIP using CRISPR. Fujita...genomic regions of interest 82613 3xFLAG-dCas9/MSCV-EGFP Expresses 3xFLAG-dCas9 in mammalian cells for enChIP...