...Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575...application, SNAP-tag, CLIP-tag (both 182 aa), and eDHFR (159 aa) are smaller than HaloTag (295 aa) – for... the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab...lab reported another AGT-based tag named CLIP-tag, which reacts with O2-benzylcytosine(BC) ligands (Gautier...Although based on the same original enzyme, SNAP-tag and CLIP-tag show good specificity for their own ligands...genetic tag on the protein of interest. Table 1: Summary of popular self-labeling tag systems Tag Size...aren’t as many commercial options for CLIP-tag and very few TMP-tag or tetracysteine labeling dyes.  If ...

...non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...Plasmid SNAP-tag Control for cell surface localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone...Plasmid CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control...or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based on the acyl carrier protein...these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty ...pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)-...ACP/MCP-tag Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and...

... segments into its own (highly Tweetable!) audio clip. In the future, you’ll be able to access these snippets...John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our Segment...

... with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene... et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National Academy of Sciences111.36... circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553....

...using a simple, external video recorder and a mic clipped to her shirt. She rolled several long takes, and...record into an external recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have...leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over in videos...: If you’ve ever watched any behind the scenes footage in the last several years, you can usually see ...

...terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG...has recovered from the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This...

...microscope stage. If using a slide, you can secure it into place using the metal clips on the stage. Turn ...Turn on the power source and use the stage arm to move the stage so that the light shines onto your sample...Once your sample is in focus, use the stage arm to move the stage (and ultimately your slide) until your... As the name suggests, light microscopes take advantage of the physical properties of light to detect ....com. Base Light Source Condenser and Diaphragm Stage Objective Lenses Focus Knobs (Fine and Coarse) Nosepiece...Slides (or other sample that can fit on a microscope stage) Reagents None needed Procedure Set up your microscope...the lowest power objective is directly over the stage. It is best practice to start with the lowest power...

...Roux lab has deposited HA tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal fusions...for an individual tagging event. Commonly used sequences, including fluorescent tags, a floxed Cbr-unc...proteins are tagged with Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly ...favorably to those of CLIP, the gold standard for characterizing RBP targets. One key advantage of TRIBE is the...technology. To streamline CRISPR/Cas9-based genetic tag insertion in the C. elegans genome, Erik Jorgensen...119 selectable marker, and “connectors” that link tags to the targeted gene are supplied to the reaction...editing in worms to the point that high-throughput tagging projects can be performed. Schwartz, et al. Genetics...

...microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect...) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the localization...autophagosomes and autophagic bodies in yeast. A GFP-tagged version of LC3, the mammalian Atg8p homolog, can...emission spectra. DsRed is the permanent fluorescent tag portion of Rosella: it will emit red fluorescence...identify because their contents can be at various stages of degradation. Additionally, electron microscopy...DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). They are connected by a 9 amino...

...generate one molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified ...connected to a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). In the absence of autophagy, the...

...resistance genes, selectable markers, promoters, origins, tags, and certain ORFs. Their sophisticated detection... between the two locations and send it to the clipboard for copy and paste functionality. Sequence ...between the two locations and sends it to the clipboard for copy and paste functionality. Restriction...

... (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent...include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed... not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus... EM, Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . ...useful (if not more so) for research applications, eclipsing past genome engineering technologies like zinc...

...monitoring autophagy A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring...categories of biosensors or browse all the biosensors tagged in our catalog . Use the article links to find ...by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy. 2007;3(5):452-60. Tamotsu Yoshimori...Hoffman Voltage JEDI-2P voltage indicator for two-photon imaging Sustained deep-tissue voltage recording...Rafael Yuste Voltage Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium...fluorescent voltage sensor. Nat Neurosci. 2014 Apr 22. Michael Lin Voltage Fluorescent voltage reporters...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters...

...cousins of well-known Cas9. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA rather than ...editing.  Find Cpf1 Plasmids at Addgene Potential advantages of Cpf1 Type II CRISPR systems based on Cas9 ...nt upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18... 18-23 bases away from the PAM. Cpf1’s staggered cleavage pattern opens up the possibility of directional...with 24 nt guides), so it has the potential to eclipse any of the CRISPR nucleases in AAV-based applications...

...prepronociceptin PPNOC POMC proopiomelanocortin ACTH, CLIP, LPH, MSH, NPP, POC PPARA peroxisome proliferator-activated... adds a myristoylation sequence and flag-epitope tag to each ORF from the labs of William Hahn and Jean...early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage tumors-like...PAFR PTGDR prostaglandin D2 receptor (DP) AS1, ASRT1, DP, DP1, MGC49004 PTGDS prostaglandin D2 synthase..., PGDS2 PTGER1 prostaglandin E receptor 1 (subtype EP1), 42kDa EP1 PTGER2 prostaglandin E receptor 2 (...MGC27302 PTGER4 prostaglandin E receptor 4 (subtype EP4) EP4, EP4R, MGC126583 PTGFR prostaglandin F receptor...prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue receptor - GIP gastric inhibitory polypeptide...