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  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...cell death. ccdA, another gene found in the ccd operon, codes for the antitoxin protein (CcdA) that protects...the cell against the toxic CcdB. Cells that lose ccdA through the loss of the F plasmid, succumb to the...gene and were therefore unable to propagate because CcdA wasn’t available to counteract the toxin. If, however...strains) will also be resistant to CcdB, as the native ccdA will be present. Most common cloning strains of ...

  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... for Using Pooled Lentiviral CRISPR Libraries” (McDade et al., 2016). To learn a bit about how CRISPR/...CRISPR Plasmids and Resources Page. References McDade JR, Waxmonsky NC, Swanson LE, Fan M (2016) Practical... Joel McDade...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...toxin-antitoxin system consists of CcdB (toxin) and CcdA (antitoxin) where CcdB inferes with DNA replication...results in DNA breakage and cell death. Conversely, CcdA binds to and blocks the activity of CcdB. Applications...and their applications MazE/MazF Aside from CcdB/CcdA, there are many protein-based toxin-antitoxin systems...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...with other deaminases: AID, CDA1, and APOBEC3G (Komor et al., 2017). CDA1-BE3 and AID-BE3 edited Cs following...activity Komor et al., 2017 BE4 and BE4-Gam; AID, CDA1 and APOBEC3G BE3 variants   A second copy of UGI...

  5. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...reagents, including many gRNA-containing plasmids (McDade et al, 2016). Many of the gRNAs contained within... Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky NC. Emerging CRISPR Technologies Available...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...manuscripts (Ersing et al., 2023; Haery et al., 2019; McDade et al., 2016), video, educational resources, and...www.frontiersin.org/articles/10.3389/fnana.2019.00093 McDade, J. R., Waxmonsky, N. C., Swanson, L. E., & Fan...

  8. Video Library

    Type
    Protocol
    ...to expand their international reach. Joel McDade Joel McDade, Addgene's Business Development Manager, ...

  20. Bacterial Expression Systems

    Type
    Collection
    ... Series Various Plasmids pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range vectors for expressing...
Showing: 1 - 20 of 24 results