...plasmid replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication...antisense RNA called Inc, also encoded by the ColIb-P9 plasmid, is complementary to part of the sequence... stringently controlled plasmids pSC101 and ColIb-P9. pSC101 copy number is regulated by iterons. RepA...called "handcuffing," which blocks replication. ColIb-P9 copy number is regulated by an lncRNA. RepZ recruits...). Copy number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense...