...of our sample libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and ...high throughput DNA prepping by trying out NEB’s new NEBNext Ultra II FS DNA Library Prep Kit for Illumina...about what I learned from a New England Biolabs (NEB) hosted workshop at Lab Central and discuss why these...Illumina sequencing and took a class at NEB’s new teaching space at Lab Central to learn all about it. LabCentral...strands can form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and...of DNA i.e 100 pg. At the start of the workshop, NEB gave a brief presentation describing the technology...were analysed to see how well the kit worked. As NEB hoped, we found that regardless of how much DNA we...

...that is only helpful for NEB restriction enzymes since the instructions use NEB buffers. Nonetheless, if... tool for someone starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot...enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when I use protocols that... if you are setting up a double digest with NEB enzymes, the app is quite helpful and provides a list ...optimal PCR depending on the primer sequence and the NEB polymerase used. Overall, while much of the information...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource ...

...overhangs with either 0% or 100% GC.) In fact, the NEB® team’s data showed that high-fidelity reactions ...these tools, NEB successfully performed a 35-fragment Golden Gate reaction, using four-base overhangs,...DAD made an incredibly complex reaction possible, NEB also optimized the thermocycler protocols and made...is the 24-piece lac operon assembly deposited by NEB – and don’t forget to ask DAD for some help! References...and overhang sets: NEBridge Ligase Fidelity Viewer™; NEBridge GetSet™; and NEBridge SplitSet™. With these... BsmBl-v2, 1x NEBridge Ligase MM 42-16 °C cycling 4-base BspQI, 1x NEBridge Ligase MM 42-...high-complexity Golden Gate Assembly reactions. NEBridge Ligase Fidelity Viewer This tool allows a user...

...fusion protein plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys” as the bacteria... achieved by inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now.../ml final). Both rabbit (EMD, AB3596) and mouse (NEB, E8032S) antibodies against MBP are commercially ...

...correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly systems) to produce... fidelity studies conducted by Potapov et al. at NEB (1, 2). While T4 DNA Ligase, the mainstay of most...be taken to minimize this possibility. Recently, NEB researchers profiled the comprehensive fidelity of... and synthesized. In conjunction with BsaI-HFv2 (NEB #R3733), re-engineered to provide improved Golden...Learn the basics of Golden Gate assembly Check out NEB's work on ligase efficiency Learn about other plasmid...

...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology...source (PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued the ACP-tag/MCP-tag product line...

...In-Fusion Cloning Design primers for Gibson Assembly and NEB HiFi DNA Assembly  OligoAnalyzer Tool from IDT: For...dimer estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD): This tool designs...cloning, Gibson assembly, or In-Fusion cloning.  NEB Ligation Calculator: This tool works for any cloning...aligns, digests, and translates a sequence.   NEBCutter2: For finding restriction enzyme cut sites Webcutter...

...containers, which is associated with CO2 emissions. NEB takes back expanded polystyrene boxes for free (in... the United States Postal Service picks up mail. NEB actually started this program in 1976! Millipore... the environment? Well, you could: Collect the NEB and/or Millipore Sigma boxes from your lab or department...

...Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) Cold Fusion Cloning (System Biosciences...of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be mixed yourself (e.g. see the...that contain the appropriate homologous sequences. NEB recommends an overlap of 15-40 bp, with a primer ... temperature greater than 48℃. Both Snapgene and NEB have tools that help you design primers for PCR amplification...

... Golden Gate Assembly with NEB's DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion...

...Addgene should propagate your plasmids in: DH5α, NEB Stable, ccdB Survival, Pir1, or other. Whenever possible...lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids containing the ccdB gene...

... high-throughput profiling New England Biolabs® (NEB) researchers recently published a method for the ... optimization method has been used internally at NEB to screen additional ligases, conditions, and formulations...an appropriate incubation temperature when using NEB thermostable ligases and reaction buffers. Many ...assembly methods, such as Gibson Assembly® and NEBuilder® HiFi DNA Assembly, require nick-selective ligases...

...transfer plasmids into bacterial strains, like NEB Stable. NEB Stable competent cells lack RecA, a protein...

... comments. Currently you can find protocols from NEB, OpenWetWare (see below), and now Addgene. I just...from external vendors, such as Nature Protcols and NEB. Quartzy Website: https://www.quartzy.com/ Quartzy...

... E. coli K-12 strain. F- λ- ilvG- rfb-50 rph-1 NEB Stable   For cloning into and storage of lentiviral...OpenWetWare's E.coli genotype resource. Additionally, NEB has a great list of genetic markers for your reference...

... his own unique way of funding basic research at NEB and got some applause when he talked about how he...

...England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer 1 NEB #B7001S NEB buffer 2 NEB #B7002S T4 DNA...DNA ligase NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012...concentration, use 1 μL) 2 μL 10x NEB T4 DNA ligase buffer 1 μL NEB T4 DNA ligase add ddH 2 O to bring...catalog # DNA Miniprep Kit Qiagen #27104 EcoRI NEB #R0101S NcoI NEB #R0193S Agarose Sigma #A9539 D.2 Screening...enzyme manufacturer's resources, such as NEB’s online tool NEBcloner (Link opens in a new window) , to help...: 5 μL Forward oligo 5 μL Reverse oligo 5 μL 10x NEB buffer 2 35 μL ddH 2 O Incubate for 4 minutes at ...TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI add ddH 2 O to bring to 50 μL ...

...viral production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids ...

...restriction enzymes, visit (Link opens in a new window) NEB . Restriction enzyme digestion is commonly used in... in a new window) double digest finder tool from NEB . In a 1.5mL tube combine the following: DNA Restriction... sticky ends. See (Link opens in a new window) NEB's compatible cohesive ends chart . If you cannot find...restriction sites. See (Link opens in a new window) NEB's table of methylation sensitive restriction sites...concentration. Learn more at (Link opens in a new window) NEB's website about star activity . If you are digesting...

...Addgene should propagate your plasmids in: DH5α, NEB Stable, or ccdB Survival.­ Whenever possible, we ..., retroviral, and AAV plasmids, we recommend the NEB Stable strain. For plasmids containing the ccdB gene...