...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed ...PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries ...take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available...

...Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et...producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. Many split-FP...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang mNeonGreen3 mNG3K...protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang sfCherry3 Improved...visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared...protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021...

... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors...Factors (MORF) Collection You may also like... Feng Zhang Lab Plasmids The Human TFome Library Stem Cells...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link...Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor open ...

...resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A....which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...A., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., ...

...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding...new. Now, Konermann and her colleagues including Zhang and Mark Brigham have taken the technology to another...literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available in Addgene...introducing the optogenetic tool, Konermann and Zhang showed they could package LITEs in viral vectors...specific epigenetic modifications in the genome,” Zhang said in MIT’s press release. In an accompanying ...

... for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4 (Link opens...

...example, the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the role of data and materials repositories...

...with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow scientists ...more Q&As which focus on genome editing using the Zhang lab's CRISPR/Cas plasmids. Designing your CRISPR...Double nickase' is a new system, developed by the Zhang lab, which has comparable efficiency to the optimized...nickase system, including one recently from the Zhang group. Learn more here. Q2: When designing oligos...without the G. Because the G is only one base, the Zhang lab usually adds it when they order the oligo. If...

... Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT and Harvard and...depositing scientist – stop by our booth to say hi! Zhang was awarded one of this year’s SfN Young Investigator...

...and budding and fission yeast. Target Finder (Feng Zhang lab) Identifies gRNA target sequences from an...non-model invertebrate species. Forums and FAQs Feng Zhang lab: Tips for CRISPR design, homology directed...cloning . CRISPR Discussion Group (moderated by Feng Zhang lab): Forum/FAQ Drosophila: General Advice and...pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9...Cas9 (pX330) or Cas9 D10A (pX335) ; PDF 248.6 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids:... psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled library amplification 1 vector system...

...screening - An excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production...using CRISPR/Cas Systems Forum- Moderated by the Feng Zhang lab, this is a useful place to ask experimental...Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional...

...screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...titer due to the smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single...Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12. (PubMed). Genome-Wide Recessive...

...in December, Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they ...perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling...enabling genome-scale, knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi... Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015 Jan 29;517(7536):583-8. doi: 10.1038...

...: 237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics... 28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins and ...electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et al., 2016). Optogenetic neuromodulation...neural activity (Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins...

...loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4. (Link opens...

...Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang Lab's CRISPR Frequently ... author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a list of FAQs about using...author of one of the seminal articles from Dr. Feng Zhang's Lab ( Science , February 2013 ), has assembled...Frequently Asked Questions You may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, ...about using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design ...Double nickase' is a new system, developed by the Zhang lab, which has comparable efficiency to the optimized...nickase system, including one recently from the Zhang group . Many protocols have been published with ...

...little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple ...a smaller, but equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over...compare the off-target effects of SpCas9 and SaCas9, Zhang’s group used an approach called BLESS (direct in ... test the efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct... Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR... Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13. Science ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014) Crystal Structure of Cas9 in ...

...  Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform called SHERLOCK...

...names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene...Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely...

...study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future, it ...