...red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579 (red) 464...separate timers shown in the table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer....7 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT 402 (blue), 583 (red) 465...red) 2.6/4.6 28 h (red) pSlow-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent...Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red) 14.9...monomeric FP timers (mK-GO from Tsuboi et al. and the FT series from Subach et al.) were derived from previously...Subach et al made a concerted effort to develop their FT series using their knowledge of protein structure...

...Monomer pFast-FT-N1 - Mammalian Expression pTRE-Fast-FT - Mammalian Expression pBAD/HisB-Fast-FT - Bacterial...pMedium-FT-N1 - Mammalian Expression pTRE-Medium-FT - Mammalian Expression pBAD/HisB-Medium-FT - Bacterial...Monomer pSlow-FT-N1 - Mammalian Expression pTRE-Slow-FT - Mammalian Expression pBAD/HisB-Slow-FT - Bacterial...) Brightness pKa Maturation Structure Plasmids Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(blue...Bacterial Expression Medium-FT 401(blue), 579(red) 464(blue), 600(red) 18.4(blue), 5.8(red) 2.7/4.7 3.9 ...Bacterial Expression Slow-FT 402(blue), 583(red) 465(blue), 604(red) 11.7(blue), 4.2(red) 2.6/4.6 28 hr (...

...their activation. The four main FTs are called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift...switchable (e.g. rsEGFP, Dreiklang). Fluorescent Timers (FT): These proteins change their color over time. Therefore...