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  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...GFP derivatives, but are instead related to the dsRed protein isolated from Discosoma sp. Similar work...Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim contributed to the writing of this article...
  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...dual colour-emission biosensor Rosella consists of DsRed connected to a pH-sensitive variant of GFP (SEP)...Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while SEP fluoresces...environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can be targeted...interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular Medicine has recently...independent gRNAs, each chosen strategically to maximize the likelihood of creating a functional knock-...
  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...the induction of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre...Timer protein - a mutant of the fluorescent protein dsRed that changes irreversibly its color from green to...Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry has developed a ...Multisite Gateway compatible destination vector for a maximum of 24 elements assembled into a final transgenesis...clonogenic neural stem cells concomitant with activated DsRed2 expression. Brown et al., Genesis 2014 Jan;...
  4. Cre-lox system

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    Collection
    ...recombination to maximize label diversity Mammalian Megason 13769 pCALNL-DsRed Cre dependent DsRed expression... pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Cre activates your gene fused to eGFP, removes dsRed. See article...Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch plasmid Mammalian Green 22799 Ai9 Rosa26...pLV-CMV-LoxP-DsRed-LoxP-eGFP Switches from DsRed to eGFP expression upon the presence of Cre Lentiviral van Rheenen 28304 pAAV-FLEX-GFP...
  5. CRISPR References and Information

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    Collection
    ...vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF 105.8...mulatta). Cas-Designer searches for targets that maximize knockout efficiency while having a a low probability...dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92 , pAC93...donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA...
  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors ...Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian Expression... 50% of its maximum value. Maturation is the time for fluorescence to obtain half-maximal value. Structure... Expression DsRed2-C1 - Mammalian Expression DsRed2-pBAD - Bacterial Expression mApple 568 592 37 6.5 ...Color) For each table: Excitation and Emission maximum are listed in nm. Brightness is the product of ...
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