...Plasmid 1: B. subtilis knock out and knock down libraries. Listen to Our segment on the B. subtilis libraries...you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production... b. subtilis...to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital Media Production Specialist at ...

...introduced into the B. subtilis genome. Mobile-CRISPRi is then transferred from B. subtilis to the bacterium...such as a B. subtilis, or a narrow range of bacterial species, such as the human gut bacteria B. thetaiotaomicron...bi-parental mating. Following excision from the B. subtilis genome, the Mobile-CRISPRi ICE element forms ...integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes needed...integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID: 27381852...

...recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin... the podcast Segement on the pPSU Plasmids B. subtilis knockout and knockdown libraries The Gross ...CRISPRi Essential gene Knockdown library. Although B. subtilis has a fully sequenced genome, many applications...used to advance our collective understanding of B. subtilis gene functions and interactions. The single-gene...-gene deletion library is derived from the B. subtilis 168 strain and contains knockouts for all 3984 ...genes identified or proposed to be essential in B. subtilis 168. Each individual strain includes a xylose-inducible...27238023 Listen to the podcast segment on the B. subtilis libraries Transcription factor fusions elucidate...

...proteins. pCri vector pCri-13a, designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence...Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids...than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory...

... Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid...

...lacO and lacO variants that can be expressed in B. subtilis Various combinations of N and C-terminal Strep...to express a protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use the same restriction...

...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. ...

...RapID, scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster...30676735. Kim, D. I., Jensen, S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (...

...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans...

...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...

...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Sciences 113.13 (2016): 3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated from a ...

...j.plasmid.2023.102684 Shareck, J., Choi, Y., Lee, B., & Miguez, C. B. (2004). Cloning Vectors Based on Cryptic... T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L....possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...