...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250...42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other...

...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description...

...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Link...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

...plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique ...

...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional Cas9 enzymes designed to introduce a double-strand break (DSBs) at a specific location based on a ...pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you have suggestions...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...pinpoint genes linked to parasite virulence, transmission, and development. This collection brings together...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

...in vivo transcrition controlled by the regulatory sequences of an RNA polymerase III transcribed U6 snRNA...can be efficiently generated in Drosophila and transmitted through the germline. Using an injection approach...

...controlling the number of mismatches we can obtain a range of expression levels. This collection comprises ...Together, these plasmids allow to explore the whole range of expression levels for these two reporters simultaneously... S. (2018). Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial...

... different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...generate single base pair edits without double stranded breaks. Cytosine base editors are created by fusing...creating a C to T change (or a G to A on the opposite strand). Likewise, adenine base editors have been engineered...

...the academic community, the labs of Keith Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger... NOTE: All zinc finger arrays listed show transcriptional activation activity of three-fold or [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. References...

...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid..., Cherfils J, Dacks JB, Lambright DG, Lee FS, Randazzo PA, Santy LC, Schürmann A, Wilhelmi I, Yohe ME...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...targeting vector that encodes both a guide RNA transcript and a repair template for an individual tagging...

...activity. Early tools allow scientists to regulate transcription, and additional tools are being developed to...Principal Investigator Plasmids GFP GFP-dependent transcription factors Connie Cepko See Plasmids Dronpa Optical...

...information. Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones...Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry Greens Plasmid Description...