...biosensors for multiscale imaging. Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha Multiple FAST Fluorescence-activating...cases an FP(1-10) fragment may be compatible with multiple FP(11) fragments, even from a different base FP...EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent...Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent...mRuby4 mRuby4(1-10), pairs with sfCherry2(11) Multiplexed labeling of cellular proteins with split fluorescent...

...developed a novel method for the easy assembly of multiple linear DNA fragments (Gibson et al. , 2009). Regardless...Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single.... Number of fragments assembled simultaneously Multiple fragments can be assembled in one reaction. However...

...consider using a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn...pipette ready, push down the plunger. There will be multiple “stops” on the plunger, where you feel resistance...

...single-color labeling; multicolor labeling would enable the visualization of multiple loci. Such a system... that uses fluorescently labeled Cas9 to label multiple genomic loci in live cells. While other systems...synthetic scaffold that can be used to recruit multiple protein molecules (and also available from Addgene...excited to see what’s coming next! References Multicolor CRISPR labeling of chromosomal loci in human ...

...downregulate multiple genes at once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac...Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent stem cells CRISPR-... deliver uniform and sustained expression of multiplexed gRNAs in human pluripotent stem cells. Previously... construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific Reports Find the...

...genes. TIP: Addgene recommends that you select multiple target sequences for each gene. Some sequences...aliquot the media into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting Target... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... SA et. al. RNA . 2003. 9(4):493-501. PubMed . Multiply attenuated lentiviral vector achieves efficient...

...remedy this, throughout the year we rolled out multiple changes to our website, including the recent homepage... new website search. Tackling findability from multiple angles    We assembled a cross-team team, with...interface.   The team approached the issue from multiple angles: the overall user experience of our website... new search tool, are the results of this multidisciplinary effort and cross-team collaboration (thanks..., human synapsin, hSynap. Matching We capture multiple variations of the same information in our search...

...Nagai laboratory has assembled a collection of multicolored nano-lanterns for use in various applications...photobleaching/phototoxicity, they successfully imaged multiple subcellular compartments over several minutes ...appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires a linear...Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.” Proc Natl Acad Sci U S... of bright luminescent protein for real-time multicolour bioimaging.” Nat Commun. 7 (2016): 13718. PMID...

...individual (and multiple) genetic perturbations in single cells. Methods to scale up multiplexed CRISPR systems...products are a diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid...characterize combinatorial gene knockouts generated via multiplexed gRNA expression (Wong et al., 2016), in addition...PMCID: PMC4785103. 11. Wong, Alan SL, et al. "Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM...

...Plasmid Kit Multiple Lentiviral Expression System Kit (Kit #1000000060) The MuLE (Multiple Lentiviral ...combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with a compound...system facilitates the simultaneous introduction of multiple genetic alterations into mammalian cells. The ...imaging studies. The ability to simultaneously alter multiple genes via infection with a single ecotropic or...

... in detail below [2]. Multipotency: When a cell can differentiate into multiple cell types of the same...[1]. Stem cells’ ability to differentiate into multiple cell types makes them useful models for developmental...hematopoietic) stem cells are a good example of multipotent cells. Unipotency: As the name suggests, cells...ASCs) Also known as somatic stem cells, these multipotent stem cells are located in small quantities in...

...increases. Multiple inserts: There are some cloning methods that make working with multiple inserts a ...as some methods are more amenable to combining multiple DNA fragments than others. In today’s post, we...per reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced cloner...one right answer, so don’t be afraid to explore multiple approaches to find out which one works best for...

...resistance gene, allowing resistant bacteria to multiply, spreading their resistance genes throughout the...selects for resistant bacteria, allowing them to multiply and propagate their resistance genes. Our society...is hoping to combat antibiotic resistance using multiple approaches. Major restaurant chains including ...antimicrobials, and they suggest that targeting multiple resistance/virulence factors may further reduce...methods. Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria...

...REPAIR and RESCUE can function in a multiplexed manner to edit multiple RNAs. It’s clear that CRISPR RNA ...research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing systems...This change specifies the edit to be made when multiple As are present in the gRNA spacer, as ADAR will...As discussed above, the ability to edit RNA has multiple advantages, including reversibility and use in...

...labs prefer to collect and pool the harvests from multiple time points while others opt for a single harvest...transfer and packaging plasmids. A benefit of the multiple harvest strategy is that each day you are supplying...destabilize the viral envelope. One drawback of the multiple harvest approach is that it tends to result in...that lentivirus produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, it is best to...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

...mutations. Multiple gRNAs could be used to combat this problem, just as ART includes multiple drugs to ...transcriptional activation. Zhang et al. designed multiple gRNAs to target the 5’ long terminal repeat (LTR...activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well as a latent...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more ...

...within a narrower band of wavelengths to allow multiple tools, such as biosensors, to be used in tandem...receptors (GPCR) regulate cellular function through multiple intra-cellular signaling cascades. Optical methods...than traditional pharmacology techniques allow. Multiple photoswitches can be used in a single pathway ...optogenetic tool, it can also cause problems when using multiple optogenetics or biosensors in one system. There...Optogenetic tools provide a powerful way to study multiple aspects of cellular activity and physiology. However...

...Screening Using CRISPR/Cas9 Deliver Multiple gRNAs at Once - Multiplex Expression of gRNAs Generating Mouse...recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different regions of your gene...reasons to target different regions of the gene with multiple gRNAs, but the most important is that you could...target artifacts by comparing the phenotypes of multiple gRNAs. Targeting different regions of the gene...

...smaller, easily customizable vector functions in multiple species, including tobacco and Arabidopsis. Improving...β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic systems, Sahoo et al....be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, ... if needed. In total, three MCS regions permit multiple regulatory elements to be inserted, further customizing...

...affect viral vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating...rich media can improve growth and DNA yield. Test multiple, small colonies After streaking LB agar plates...cultures contain the recombined vector backbone. Test multiple colonies before saving a glycerol stock to ensure...try re-streaking for single colonies and testing multiple preps. In some cases, we suggest gel extraction...