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Showing: 161 - 180 of 248 results

  1. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases...consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base...the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated the use...design of short oligonucleotides needed to construct gRNA expression vectors. More information about RGNs ...

  2. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  3. Validated gRNA Sequences

    Type
    Collection
    ... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA Target...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences that have...Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...details and outcomes. Submit Validated gRNA Sequences Validated gRNA sequences can be added to this table...validated gRNA sequences. Genome...

  7. FlyCRISPR

    Type
    Collection
    ...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - ...template: example : Resources: Protocol for generating gRNAs 106.5 KB flyCRISPR website contains additional information...information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input sequence and checks...replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro production of RNA ...

  8. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries ...Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful...experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic ...thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...

  12. Plan Your Experiment

    Type
    Guide
    ...System Design gRNA Know your Cell Line Select Gene Select gRNA Clone gRNA Delivery of gRNA & Cas9 Validate...how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have selected...enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence ...delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then... Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are combined...modify prior to designing your gRNA, as sequence variation between your gRNA targeting sequence and target...the associated gRNAs based on their predicted on-target and off-target activity ( see gRNA design software...

  13. Lentiviral Prep Service

    Type
    Collection
    ...RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting...of Cas9 + ACE2 gRNA Sheltzer 153015 TMPRSS2 g1 + Cas9 Expression of Cas9 + TMPRSS2 gRNA Sheltzer Viral... Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro...

  16. dTAG - You're it!

    Type
    Blog Post
    ...CRISPR plasmids for protein tagging Find validated gRNAs ...

  18. Plant Plasmids and Resources

    Type
    Collection
    ...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots...

  19. Qi Lab CRISPR Page

    Type
    Collection
    ... fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either...use editing bacterial genomes 44251 pgRNA-bacteria A customizable gRNA expression plasmid for use with the...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently ...resistance. The second plasmid ( pgRNA_bacteria ) contains a constitutive sgRNA expression cassette on a ColE1...The second plasmid ( pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette, wherein ...dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair complementary region...

  20. CRISPR Guide

    Type
    Guide
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA binding induces...if the gRNA spacer sequence shares sufficient homology with the target DNA. Once the Cas9-gRNA complex...
Showing: 161 - 180 of 248 results