...in immune cells have identified many putative regulators of immunity. However, in vitro screens cannot...responses to cancer. The screen recovered known regulators, such as Ptpn2, Pdcd1, and Zc3h12a, demonstrating...Moreover, the screen identified putative novel regulators of CD8+ T cell responses in tumors, demonstrating...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...

...establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH, in the United...can be found during conversations with senior investigators. Discussions can reveal mutual scientific interests...makes time for you, as needed, this is a strong indicator that this person cares about your science and ...manuscript reviews, etc. You'll also meet future collaborators and get access to unpublished information and...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

...customized to control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target...Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of...broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et... frequently been used for screening candidate regulatory elements such as enhancers and promoters for ... its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB (Fig. 4c, Jang et al....., … Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(...

...technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods...tagging of proteins with mAID and other tags (Yesbolatova et al., 2019) using CRISPR. This toolkit makes...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...

... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https:...

...heterologous envelope or a post-transcriptional regulatory element can be used for either type of retrovirus...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...strains have been engineered to induce a lower inflammatory response within hosts. Relative to the other...

...immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...self-limiting effects kick in trying to resolve the inflammatory state by inducing immunosuppressive measures...Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is ...a researcher to control immune signaling and inflammatory responses remotely with spatiotemporal precision...

...CRISPR for that, too! CRISPR activators (CRISPRa) are transcriptional activators fused to a dead Cas9. The...increase expression. There are several flavors of activators which can be used with this approach - read more...them on our CRISPRa blog! Fig. 1 CRISPR activator and repressor mechanisms Want to transiently...

...sword – balancing antiviral benefits with the inflammatory response associated with the introduction of...threshold and thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated...its way outside of the mitochondria, the same inflammatory response can activate. These types of cellular... disorders, including some forms of lupus and rheumatoid arthritis. These types of immune responses are...

...used red fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can...expression robustly activated the reporter gene TdTomato, whose expression was absent without electroporated...T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a high activation...frequency of 56-93%. In the converse test, 98% of TdTomato expressing cells were GFP+, indicating a robust...

...available to counteract the toxin. If, however, an investigator were to successfully clone an insert into the...insertion will completely replace ccdB with the investigator’s insert of interest. Hence correct clones are... behind the “swapping out” of ccdB with the investigator’s gene of interest.   CcdB resistant E. ...for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995 Mar;15(6):1031...

...activation of keratinocytes, which release inflammatory mediators that trigger the activation of dendritic...Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6...IL-36 cytokines are a potent inducer of an autoinflammatory loop: keratinocytes both respond to and release...

... infections along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential... 1939 for the treatment of botulism and Lambert-Eaton myasthenic syndrome (LEMS) because, at high concentrations...

...bone marrow (BM) cells, primary human hepatocytes, and hematopoietic stem cells (HSCs) using Nanoblades....deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...from GFP transgenic mouse  GFP  ~60-65% Human hepatocytes  Myd88  ~50% Human HSCs  Myd88  ~50% Table...

..., 50ms “off”.  A pulse generator such as the A.M.P.I Master-9 Pulse Stimulator, which can control output...cable is threaded through—and fixed to—an optical insulator called a ferrule (below). The fiber optic cable...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

... bank account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab...business. We ended up applying to a business accelerator freely stating that we had an idea, knew how ...rough day, and we have great partners in this accelerator program who want us to succeed. We’re excited...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside...

...common use of intradermal injections is studying inflammatory responses, as this method is useful for analyzing... correct location requires a lot of skill and anatomical knowledge. Intrathecal injections are used for...Routes of administration. In H. Hedrich (Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, ...M. A. (2011). Administration of substances to laboratory animals: routes of administration and factors...

...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....nuclease into three functional groups: nucleases, modulators, and chimeric effectors. Nucleases generate a... a classic double-stranded break, while modulators regulate transcription and chimeric efforts make a ...deposited and distributed CRISPR plasmids. But modulators and chimeric effectors, made available in 2013...

...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...operations, she also works on improving laboratory efficiency through automation.       More Resource Avaliable...

...m a father of three, a scientist and science communicator, and I strive to be a volunteer and contribute...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...great feedback from teachers and other science communicators.  What can CRISPR do? I spent more time on...