...scientific expertise. Organizing a career fair for early-career scientists provided a crash course in this...I find it useful to remind myself to check in regularly with collaborators for example on a holiday or...regard to reaching out to new people, checking in regularly, and maintaining useful notes that remind me about...

...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...CRISPRa) are great ways to modulate the expression of nearly any gene via specific guide RNA (gRNA) sequences... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...

...products may be toxic to the host when expressed, particularly those that are insoluble, act on DNA, or are... can neither restrict nor methylate DNA. dcm: Similarly, strains with this mutation are unable to methylate...dead cells, and, conversely, you cannot induce too early as there are not enough cells in the culture to ...

...improve muscle function, and they must be injected regularly. Dystrophin and genome editing Since complex oligonucleotide...approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases to remove...force, and other muscle tests, muscle function is clearly improved, although not to wild-type levels. With...

...H3K27ac histone modification at specified loci. Charles Gersbach’s lab has deposited mammalian expression...pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...this article. References Haldeman JM, Conway AE, Arlotto ME, et al (2018) Creation of versatile cloning ...

...fluorescent and can be challenging to track intracellularly, but aptamers permit the study of intracellular...is better able to maintain its fluorescence intracellularly due to a lower dependency on magnesium and ...binding with RNA by about 7x). Mango fluoresces nearly as brightly as Spinach, but binds with much higher...

...inconvenient data must not be ignored. Returning to an earlier analogy, no amount of shouting will make an apple...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate...13th the March for Science homepage has amassed nearly 500,000 ‘likes’, while their website lists over...

...research directions that were inaccessible with earlier tools. They also produce more than 20 other MTRIA...MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various...brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3...

...transparent. Thus scientists can easily observe early developmental stages which can be difficult in other... large experiments over multiple generations. Similarly to other model organisms, the C.elegans genome... PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease models in Drosophila...

...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

...the description to watch any sections you’re particularly interested in, but I’d recommend watching the...Reproducibility Project: Cancer Biology have produced similarly low rates of reproducibility. Failed attempts ...modified analyses on published data if you need to. Similarly, FigShare also makes it easier to share data through...

...Controlling the expression levels of your gene may be particularly useful when the gene being expressed has threshold...transposase/transposon gene modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only...accessory transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration...

...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... get an experimental system up and running very early on. Once your system is working, you should maximize...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...

...and applied research since their first use in the early 90’s to genetically modify primary cells. Amongst...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...was obvious that its safety was a disadvantage particularly for clinical development. In addition, MoMLV...

...base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined with nearly the same efficiency...high-throughput profiling of ligase fidelity that extends earlier studies through a high-sensitivity multiplexed ...

...that other technologies need to be discarded but clearly we are in an era of neuroscience research where...where optogenetics will be a standard tool used in nearly any neuroscience project or topic of interest. ...elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of the key...

...brain along with high off-target expression, particularly in the liver, when administered intravenously...different efficacy and tropism between NHP species, particularly between New World and Old World monkeys. Selecting...but its neuron transduction encompasses more molecularly-defined neuronal subtypes and with less bias ... to AAV9 in the pigtail macaque brain slices. Similarly, AAV9-X1.1 outperformed the other capsids in the...

...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...

...been an important animal model for research, particularly genetics research, for decades. Its short life...during our stay there. Yet capturing the flies is nearly impossible, due to their small size and inconspicuous...

...brain tissue is injected with compounds that intracellularly spread away from the site of application and...transport has been observed for AAV1, -5, -8 (McFarland et al., 2009), and bidirectional transport for...Neuroscience 193, 249–258. PubMed PMID: 21782903. McFarland, N.R., Lee, J.S., Hyman, B.T., and McLean, P.J...