...107787 AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype...

...expression, as well as the substantial immune response induced by the viral capsid. Adenoviruses can be produced...

...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...

...exclusively for surface proteins and use enzymatic binding to attach substrate Control plasmids with a recognizable...

...Chemogenetics AAV Use our chemogenetics AAV to chemically induce neuronal activity in specific cell types. See our...

...closed) (only you and Addgene have access to your individual folder and your materials will not be shared ...

... collection of plasmids suitable for creating individual fluorescently tagged proteins to study protein-protein...

...Addgene's zebrafish resources: Find plasmids for use in zebrafish....

...about this field. Use the table and filters below to find ready-to-use AAV preparations of optogenetics plasmids...

... Protocols Ligation Independent Cloning Ligation Independent Cloning You...for Ligation Independent Cloning Protocols...Molecular Biology Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-...

...Validation Using the Indirect ELISA Method Antibody Validation Using the Indirect ELISA Method You may...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) against...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize...

...publically available through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869...Toolkit Many industries, such as the food and beverage industry, the bioethanol industry, and the pharmaceutical... make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from ...Nile Virus (WNV) induced cell death. The Wu lab library consists of 77,406 individual gRNAs targeting ...optimizations are required for each individual target species. Indeed, in order to use MAGE, the λ Red recombinase...for genetic optimization of industrial yeast strains. However, industrial yeast strains are more difficult...Targets of RNA-binding proteins identified by editing Identifying targets of RNA-binding proteins (RBPs...

...FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9 for genome...Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible dCas9-VP64...domain unfolds allowing it to bind to SspB. This original Light Inducible Dimer (oLID) could be used to... not show large changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold...throughput binding assays, to engineer photoswitchable dimers which show over a 50 fold change in binding affinity... coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites to tether...constitutive or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene...

...ON. When a repressor binds the activator protein, the activator protein cannot bind the promoter sequence... cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor...derivatives is added, it binds tTA, resulting in a confirmation change that prevents binding to the promoter and...cumate is added, it binds cTA, resulting in a confirmation change that prevents binding to the promoter and.... As ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1...system). The GAL80 repressor can bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can therefore...manner, with LexA binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (DBD) to GAL4...

...interactions. Next comes the binding step where the target antigen binds to antigen-specific antibodies...conjugated antibody binds to antigen coated on the multiwell plate. In an indirect ELISA a primary antibody...antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds to the primary...antibody that binds to the antigen in a sample and then a second detection antibody binds. The detection...less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA the reporter...research before conjugating at the bench.   Indirect ELISAs The indirect ELISA process is similar to that of ... easy to find,  it is often easier for labs to source appropriate antibodies for an indirect ELISA than...

...dark-induced dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced Table...controlled binding, but with opposite effects: insertion into loop 1 caused dark-induced binding while loop...OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue light inducible and dark-inducible...OptoNB can bind, it co-localizes with its target at the cell membrane and when the OptoNB can’t bind, it localizes...loop 6 insertion caused blue-light induced binding. The lab used this general design scheme to generate...the effect of AsLOV2 insertion site on light-induced binding.  Light controlled protein purification with... path to light-controlled protein binding, they probably don’t bind your favorite protein yet. More OptoNBs...

...While a primary antibody binds to the protein of interest, a secondary antibody binds to the primary antibody...primary antibody, due to increased binding affinity, many indirect antibody protocols are designed to ...anti-mouse secondary will bind to all the primary antibodies indiscriminately, giving them the same output...antibody with a signaling molecule, the antibody binds to a protein, and then voila! Wherever your protein...protein of interest, it may be time to look at the indirect approach - which means it’s time to talk about...antibody. In the indirect approach, the signaling molecule is conjugated to the secondary antibody instead...antibody. Since multiple secondary antibodies can bind to a single primary antibody, this approach can ...

...attachment. The five other IPI rAbs bind RGD-binding integrins at non-ligand-binding sites and do not interfere...morphology changes induced by integrins binding the RGD motif compared to those binding the Leucine-Valine-Aspartate-Proline...integrin antibodies exist, almost all bind outside the ligand-binding pocket, their sheer size impeding ligand... investigators inhibited other RGD-binding integrins, individually and in combination, showing that among...study. Cells often express multiple integrins that bind the same ligand, making it difficult to dissect ...The collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some...antibodies in the collection are able to block ligand binding — and therefore interrupt integrin function — providing...

...SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here! Find viral prep here: pAAV.hSyn.FLEX.iGluSnFR3...al. 2022, under CC-BY-NC license.   Find JEDI-2P plasmids here! Find viral preps here: pAAV-EF1a-DIO-JEDI...this in mind, Ed Boyden and colleagues set out to engineer a potassium ion biosensor with a binding affinity...of potassium binding proteins, they developed a family of fluorescent potassium indicators with a range...new tools to measure their favorite targets and finding new targets to measure! Let’s take a look at some...adapted from Ino et al. 2022, under CC-BY license.   Find MTRIA sensors here!   Ino D, et al. A fluorescent...doi: 10.1038/s41592-022-01597-x. New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR...

...transferred to an industry position. If you cannot get an Industry H-1B, remember that industry career paths...Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry H-1B There...process or quota like industry, these organizations can be a good choice for individuals needing sponsorship...importance of the individual’s work in their field and the impact of the individual’s past work on the ...passionate about working with scientific individuals to find exciting growth opportunities in life sciences...growing shift away from employers sponsoring individuals for green cards because of the cost, long timeline...available to you including careers in academia, industry, non-profit, and government sectors.  Within these...