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We narrowed to 238 results for: Anti-CRISPR

Showing: 161 - 180 of 238 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ...purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing... is gradually reduced. For example, after using CRISPR/Cas9 to edit a gene, the expression of Cas9 is ...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology...isolating genomic DNA from transduced cells and quantifying the integrated viral DNA along with a reference...detecting vector particle components or performing quantitative PCR titration of vector DNA in samples harvested...

  2. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post
    ...Educational Resource Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide... What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to ...primers to help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the ...to identify and reduce barriers for scientists wanting to share their tools with Addgene.  In our next...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

  3. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence...Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to study epigenetic modulation...customizable protein degradation. Enter TRIM-Away - an antibody-based system developed by the James and Schuh ...protein variants when used with a variant-specific antibody. TRIM21, an E3 ubiquitin ligase, is the basis ...recruits ubiquitin and the proteasome to destroy antibody-bound pathogens. Clift et al. found that addition...addition of exogenous TRIM21 protein along with an antibody targeting the protein of interest led to rapid...co-microinjection of TRIM21 protein and the target antibody works well. In larger cell populations, you can...

  4. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...Gene expression control Researchers are always wanting to manipulate gene expression – turn it up, turn...including 1) cellular responses in addition to antibody response 2) very high immunogenicity 3) and long-lasting...

  5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post
    ... of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with...Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...high-quality isolated DNA samples of sufficient quantity for sequencing. This process is completed in a...significant amount of QC. First, we perform Picogreen quantification on each and every isolated plasmid sample and... preparation process and includes Picogreen quantification of various intermediate steps, a sizing electrophoretic...electrophoretic gel, and qPCR quantification of the final libraries. A broader view of the whole plasmid...

  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...expression, mouse targeting, CRISPR, TALEN, synthetic biology, affinity reagent/antibody, unspecified, or other...Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment Suggest A Plasmid You'd Like to Find...other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that should...or low copy. Choose “high copy” if a sufficient quantity of DNA is produced from a miniprep and “low copy...

  7. Important Considerations When Using AAVs

    Type
    Blog Post
    ...system. Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Gene Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional ...to develop “AAV 3.0”, vectors that will have substantially improved performance profiles and should be ...microRNAs (miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger nucleases...

  8. RaPID Detection of RNA-protein Interactions

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    Blog Post
    ...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...RNAs and proteins with UV light, followed by an antibody pulldown of a protein of interest, much like using...

  9. Hot Plasmids: Summer 2025

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    Blog Post
    ... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ...months we highlight some of the new plasmids, antibodies, viral preps, and more in the repository through...fused to mVenus, mGold2s, or mGold2t. Bottom: Quantification of photostability. Image reused from Lee & ...ATLAS (anterograde transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold... Arnold Lab, ATLAS is based on an antibody-like protein AMPA.FingR (AF) that binds to AMPA receptor subunit...

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...several advantages over the alternative systems – CRISPR, siRNA, etc. Firstly, they are reversible mechanisms....1073/pnas.1216880109 Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging...with the most alternative functions – there are antibodies to it and ways to achieve fluorescence with it...Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...encodes a Type I-F CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA (crRNA), 2) ...sets the stage for combining the super powers of CRISPR and transposons to allow for the targeted delivery...For example, in bacteria, transposons can move antibiotic resistance genes between plasmids or from plasmids...

  12. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...mammals), cloning techniques, and experimental use (CRISPR, viral production, RNAi, protein expression etc...streaking to a single colony to DNA extraction and quantification so that you can focus on what you want to do... grades of DNA  Read about the various ways to quantify DNA Resources on Addgene.org Learn about our...

  13. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids. Our most...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this...which includes both neuroscience and general-use antibodies, is available in both trial- and full-size, with...

  14. AAVs in Retinal Gene Therapy

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    Blog Post
    ...trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly well suited to.... Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina. Front Cell ..., meaning that it can tolerate the presence of antigens (such as viral vectors) without triggering a destructive...

  15. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...the Inntag Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein of Interest ...expression level without the need for a custom antibody. There is one major caveat - a tag may interfere...affinity for other proteins, and accessibility to an antibody. From their initial pool of candidates, they selected...

  16. Plasmids for Stem Cell Research

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    Collection
    ...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human...enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski... Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-Mediated Differentiation...Mouse Direct reprogramming of fibroblasts into antigen-presenting dendritic cells. Sci Immunol. 2018 Dec...

  17. Depositor Collections

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    Collection
    ...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression... Plasmid Special Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid ...

  18. Allen Institute for Cell Science Plasmid Collection

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    Collection
    ... Plasmid Collection You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide...PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can...

  20. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post
    ...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...excited to launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll...world. That included 2008 pooled libraries, 290 antibodies, 18,359 viral items, and 166,247 plasmids. We... our distributor partners, with the launch of antibodies in China and expansion of viral services available... in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first IPI collection...
Showing: 161 - 180 of 238 results