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Showing: 161 - 180 of 425 results

  1. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). Figure ...doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein...synthetic biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve...1985; 43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein...membrane protein interactions. Proc Natl Acad Sci U S A. 2004; 101(33):12242–12247. Pubmed PMID: 15299147...

  3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... and resources References Aschauer DF, Kreuz S, Rumpel S (2013) Analysis of Transduction Efficiency, Tropism... OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant....org/10.1016/j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette...

  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...doi.org/10.3389/fimmu.2017.01589 Ayyar BV, Arora S, Ravi SS (2017) Optimizing antibody expression: The...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...processed miRNA or siRNAs then silence the target gene(s) either transcriptionally or post-transcriptionally...produced that contains sequence against your gene(s) of interest and the reporter. This approach greatly...Plant Biology 7:251–257 . https://doi.org/10.1055/s-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack...F, Joberty G, Zinn N, Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, ...

  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...detectable using PCR. The system also functions in S. pneumoniae and can be used to generate multiple mutations...gene lambda Red. pTargetF contains the specific gRNA(s), and the repair template is supplied as a dsDNA fragment...Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE (2018....7b00399 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-...AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating essential gene function...

  7. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated...doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (2002). Maltose-Binding Protein as a Solubility Enhancer...

  8. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, for mouse or human receptors, respectively). ...AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo brain microdialysis: advances in...

  10. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...//doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector,...10.1128/jvi.71.8.6174-6178.1997 Wickersham IR, Finke S, Conzelmann K-K, Callaway EM (2006) Retrograde neuronal...Wickersham IR, Lyon DC, Barnard RJO, Mori T, Finke S, Conzelmann K-K, Young JAT, Callaway EM (2007) Monosynaptic...

  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...between protein abundance and band intensity is an S-shaped curve. Sparse proteins may not be detected .... Created with BioRender.com. To see why this S-shaped relationship poses problems for proteins of... of this gel is misleading. We can see why on the S-shaped curve. On the protein abundance axis, both ...

  12. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...still work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in ...site directed mutagenesis to remove the BbvCI site(s). Overall, the key features of the one-pot saturation...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...to invert or ‘flip DNA’, is derived from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). The ...References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin...Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(15): 6991–95. PubMed PMID: 7624356. PubMed Central...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...Renaud J-B, Schneider-Maunoury S, Shkumatava A, Teboul L, Kent J, Joly J-S, Concordet J-P. Evaluation of...e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N, Giugno R, Pinello L. CRISPRitz...

  16. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...190500 Homer1L/S scFv [L113/130] L113/130 scFv 2a Homer1L/S Mouse Mouse 190501 Homer1L/S scFv [L113/130...Gamma2L/S scFv [N452/73] N452/73 scFv 2T GABA(A)R, Gamma2L/S Human Mouse 190556 GABA(A)R, Gamma2L/S scFv ... Human Mouse IgG2a 177456 Anti-Homer1L/S [L113/130R] Homer1L/S Mouse Mouse IgG2a 177457 Anti-Homer1L [...IgG2a 177553 Anti-GABA(A)R, Gamma2L/S [N452/73R] GABA(A)R, Gamma2L/S Human Mouse IgG2a 177554 Anti-GABA...N476/9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [...Human Mouse IgG2b 206636 Anti-Homer1L/S [L113/130R-2b] Homer1L/S Mouse Mouse IgG2b 206637 Anti-Homer1L... Human Mouse IgG1 206687 Anti-Homer1L/S [L113/130R-1] Homer1L/S Mouse Mouse IgG2a 206688 Anti-Homer1L ...

  20. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...that can be imaged with the optics on the microscope(s) you intend to use. An accurate determination of whether...protein that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red...
Showing: 161 - 180 of 425 results