We narrowed to 221 results for: ung

1. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   ...Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine...

2. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   ...nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg...

3. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   ...Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination events during integration of transfected...

4. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   ...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. ...

5. Illuminating Epigenetics with A FRET Based Biosensor

   Type

   Blog Post

   ...disabilities, problem behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. ...

6. CRISPR Plasmids - C. elegans

   Type

   Collection

   ... In vitro transcription none, need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection...

7. Synthetic Biology - Overview

   Type

   Collection

   ...Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica...

8. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   ...Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György...

9. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   ... S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity...

10. Plasmids 101: Degron Tags

    Type

    Blog Post

    ...13, 5495 (2022). doi:10.1038/s41467-022-33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper ...

11. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    ...10.1586/14737159.5.2.209. PMID: 15833050. Ma L, Chung WK. Quantitative analysis of copy number variants...

12. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ...Central PMCID: PMC4686367. 2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency...

13. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    ...non-target DNA strand and shows the HNH nuclease domain swung around in close juxtaposition to its cleavage site...

14. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    ...-assisted insertion tagging), developed by the Hornung Lab, allows users to easily create C-terminal tag...

15. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    ...females because you can be certain that they are too young to mate. Using various markers (more on that below...

16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    ... Center did to create a model of Eml4–Alk-driven lung cancer (Maddalo et al., 2014). These tools are available...

17. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ... Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. ...

18. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...adapted to work in prokaryotic bacteria, not in fungal systems or plants. We are really plugging into ...

19. AAV for Neuronal Tracing

    Type

    Collection

    ...DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. 2007b. Monosynaptic restriction...

20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    ...peripheral nervous system (PNS), which connect the heart, lung, gut, and other organs to the CNS, are also an important...