Rabies and Neuronal Tracing
Type
Blog Post
...development or that blood flows through the heart and lungs and oxygenates the body. However, in neuroscience...
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Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post..., Wood, V., & Fantes, P.A. An ancient yeast for young geneticists: A primer on the Schizosaccharomyces... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...genes are important for SARS-CoV-2 entry into human lung epithelial cells (Biering et al., 2021). In their... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post... markers. New CRISPR pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...Murnane, John P., Michael J. Yezzi, and Barbara R. Young. "Recombination events during integration of transfected... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. ... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...disabilities, problem behaviors, and a chronic feeling of hunger that can lead to excessive eating and obesity. ... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post... S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity... -
Plasmids 101: Degron Tags
TypeBlog Post...doi: https://doi.org/10.1038/s41467-022-33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper ... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...non-target DNA strand and shows the HNH nuclease domain swung around in close juxtaposition to its cleavage site... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...-assisted insertion tagging), developed by the Hornung Lab, allows users to easily create C-terminal tag... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...females because you can be certain that they are too young to mate. Using various markers (more on that below... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...Central PMCID: PMC4686367. 2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post... Center did to create a model of Eml4–Alk-driven lung cancer (Maddalo et al., 2014). These tools are available... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...10.1586/14737159.5.2.209. PMID: 15833050. Ma L, Chung WK. Quantitative analysis of copy number variants... -
Light Sheet Fluorescence Microscopy
TypeBlog Post... Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. ...