Progress Towards a PAM-Free CRISPR
Type
Blog Post
...gkab507
Resources on Addgene.org
Addgene’s CRISPR Guide
CRISPR Plasmids and Resources
Resources on the ...Cas9
xCas9: Engineering a CRISPR Variant with PAM Flexibility
History of CRISPR Cas – A Tale of Survival...sequence for your genome engineering experiment. With CRISPR entering the clinic for correction of disease-causing...landscape.
The need for PAM-less editing
Guides for CRISPR are typically selected by the ‘quality’ of the ...available PAM must be selected.
When it comes to CRISPR experiments, therefore, you often must make a choice... all genomic landscapes should be editable with CRISPR technology.
Fig. 1: SpCas9 has sparse...Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368,290-296(2020). DOI:10.1126...