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Addgene
Showing: 141 - 160 of 215 results
  1. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...catalogue numbers and manufacturers’ details for antibodies, plasmids (like your Addgene plasmid #), and ...an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software tools in the Materials...
  2. Plasmid Collections

    Type
    Collection
    ...γ-Retrovirus Adenovirus All Viral Plasmids Curated Topics Antibody Plasmid Collection Bacterial Expression Systems...
  3. Resolute Plasmid Collection

    Type
    Collection
    ... production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an...
  4. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for...
  5. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...are easily studied in living cells using antibodies. Antibodies could further be used to interfere with...with the identified surface proteins, and such antibodies could thus be interesting drugs. Known drug targets...
  6. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses ...
  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...activity (Ni, Titov, & Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013),...synthesized with reagents used to fluorescently label antibodies, and purification is a snap using thin layer ... et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS ...
  8. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...with the most alternative functions – there are antibodies to it and ways to achieve fluorescence with it...Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...
  9. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells...types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia are...
  10. Hot Plasmids - August 2020

    Type
    Blog Post
    ... The benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are smaller in...easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed in the cytosol...
  11. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...additional data regarding the specificity of this antibody?” You will also help troubleshoot products, give... in a Western Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat...
  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...Fluorogen activating proteins (FAPs) – These modified antibody fragments bind and enhance small molecule fluorogens...Nevertheless, these fluorophores were designed as antibody and oligonucleotide labels for fixed-cell imaging...
  13. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended...
  14. CRISPR Plasmids - Yeast

    Type
    Collection
    ..., and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design ...
Showing: 141 - 160 of 215 results