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We narrowed to 349 results for: Plat

Showing: 141 - 160 of 349 results

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...repair, as there is no requirement for a repair template (sister chromatid or homologue) or extensive DNA...NHEJ-dependent repair (“pop-in” insertion) provided a template containing compatible overhangs is available. ...

  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... not limited to: rapid growth, ease of replica plating and mutant isolation, a well-defined genetic system...complementation is weak. yes   LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...relevant host biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR... al. "A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers...

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...use every single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise...

  7. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...percent of cases, the stem cell will seek out a template for the repair process. By flooding the cell with...using the introduced donor plasmid as a repair template. This results in the insertion of the GFP tag ...

  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen, 2016...PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic acids...

  11. Hot Plasmids - October 2020

    Type
    Blog Post
    ...requires the much less efficient double stranded templates, rather than the more efficient single-stranded...cellular protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less...

  12. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture volume into which the ...vector using the harvested viral solution as a PCR template. Regardless of the type of virus you’re working...

  13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ... have members looking at how to re-use it as a platform for monitoring homemade microfluidic chips using...using the fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly...

  14. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...signaling in primary cells. The authors used this platform to assay dozens of GPCRs across many cell types...context-dependent GPCR activity with a universal biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org...

  15. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Cutting both strands ensures that there isn’t a template strand left behind that the cell can use to repair...combination of chewing up one stand and cutting the template strand causes large deletions of up to hundreds...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that the ...plasmid, not that I would know about this… A no template control (NTC) should always be included. While...

  18. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... on Friday. It’s best to split cells before the plates are more than 80-85% confluent because cells like...Benjamin Franklin)! It’s good practice to label your plates and/or flasks with your name, date, the cell line...

  19. PhD Applications After COVID

    Type
    Blog Post
    ...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...
Showing: 141 - 160 of 349 results