Isolating a Monoclonal Cell Population by Limiting Dilution
Type
Protocol
...Corning 430167 (optional) Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-L-glutamine (or...Each 10 cm dish should be seeded in 10 mL DMEM complete, which will generate enough conditioned medium...conditioned medium, prepare this cell solution in fresh complete medium. This 5 cells/mL solution will be used ...adding 10 µL homogenized cell solution to 990 µL complete medium. Instead of transferring 0.125 µL, now ...