...ambitious projects that require epitope tagging of a large cohort of genes (e.g. tagging genes representing...success of the epitope tagging experiment depends largely on how efficiently these components are delivered...immense possibilities to delineate the function of large protein cohorts in diverse mammalian cell types....

... chemical synthesis of these molecules. To make large amounts of pure product in an affordable way, researchers...that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016...needed in mammalian cell types, and comes with a large library of common parts to get you started quickly...build genetic circuits and would benefit from a larger library of parts but don’t need to add tags to ...multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those...

... a patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different...

... the user in different formats. This can have a large impact on downstream results. Examples: “HTML”, ... agnostic”, “model organisms”, “small genome”, “large genome”, and “organism biased scoring”. Trends: ... CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile buying a dedicated...

...deliver larger cargo such as base editors and prime editors. While using AAV to deliver large transgenes... with split AAVs. In general split AAVs break a large transgene in two pieces and package each piece into...

...offering temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at...expertise in a specific country can quickly reduce a large number of barriers. Though we haven’t solved every...

...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...

...Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested in science... moderators did not delete or suppress were the large number of scientist posters with serious career ...

... to its efficient use in screening experiments; large amounts of virus are required to ensure that you...

...open-access. Though it is time for me to refill my large coffee cup and get back to our booth, I will have...

...colonies that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive...other methods. This could be a reflection of the large size of the plasmid or silencing of the plasmid ...oncogenes, such as Myc. Viral vectors also have the largest genomic footprint of iPSC generating methods due...infect dividing and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral...transfection efficiency is cell-type dependent and larger plasmids have lower rates of transfection. Minicircles...

...destined to continue in academia. It seems it’s largely a matter of luck if a student or postdoc ends up...

...transformed without a heat-shock step. Because of the large number of transformations being performed at Addgene...

... possible to quickly and efficiently assemble a large number of building blocks with just two or three...

...individual plasmid within a single bacterial cell. Large plasmids usually have a low copy number (approximately... other hand, smaller plasmids can be present in large numbers, 50 or more per cell and have a high copy...usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much as a liter ...

...counter-selection. Anecdotally, there is also a large amount of user-to-user variability, and in some ...modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise point mutations...

...companies now sell fast digest enzymes that can digest large amounts of DNA in as little as 10 minutes, but check...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ..., the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep...

...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...

... from 10-50 µL depending on application and is largely determined by the volume of DNA to be cut. A typical...website about star activity . If you are digesting a large number of plasmids with the same enzyme(s) (for ...

... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...