...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional... test and discover what career suits you best I began viewing my activities as types of experiments. I...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...graduate experience, the point at which my career path began to take shape, was when I started volunteering. ...

... priority and have organized many events over the years. To keep the organizational side of things from...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...event! Addgene company culture At Addgene we organically developed the philosophy of  “work hard, play... in getting the fun started. I’m aware that  “organizing the fun” sounds a little neurotic, but hey- sometimes... her operational responsibilities, she enjoys organizing team building events at Addgene and cultivating...

...Williams, Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland...discussion with my dinner partners Denise Akob and Geof Hannigan that the outline for Binning Singletons came together...a positive stir and was recognized by meeting organizers in their town hall.  Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...Eric Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral...

...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...important part of the application. Even within an organization, there can be variance. At Addgene, our content...explain why you are interested in working for that organization or group. This is particularly important for...want to talk about! You could describe your organizational system, your rationale for designing it the...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...that particular job, your commitment to the organization's mission, or your deep interest in the lab's...

...orphan receptors meaning their ligands are unknown. Identifying new ligands for orphan GPCRs is of significant...Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized genome editing in multiple organisms thanks ...construction of a so-called “genomically recoded organism” (3) but its portability to other bacterial strains...monitoring and analysing autophagy of cytosol and organelles in yeast cells. Named after the brightly-coloured... both in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly...screens required to identify correctly modified organisms has limited routine use of this technology. To...CRISPR/Cas9-based genetic tag insertion in the C. elegans genome, Erik Jorgensen’s lab from the University...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....them is one of the strongest noncovalent protein-ligand interactions found in nature. Once the bond is ...is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...these features it isn’t surprising that scientists began to exploit biotin and its strong interaction with...

...transducer complexes that are activated in response to a ligand binding a GPCR of interest. How does TRUPATH work...intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the GPCR causes...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...emission. Following activation in response to the ligand, the complex will disassociate, resulting in a ...across the transducerome for a specific receptor-ligand pair. They further used TRUPATH to analyze a panel...This collection can be used to study endogenous ligands, the pathways they activate, as well as the activation...

...designer ligands that can be simply introduced by injection. The main challenge in using ligands is ensuring...at broad tissue or system-wide targeting, as the ligand can reach target cells throughout the body. Controlling...chemogenetics, while different concentrations of ligands can be injected, this is not as precise and the...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...while also being selective and effective. A new ligand from the Bryan Roth lab and Takafumi Minamimoto...

...nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like... about a local food pantry or community arts organization. Many people are surprised to learn that Addgene...nonprofits do with their revenue. A nonprofit organization cannot distribute profits to private individuals...as a nonprofit, we attract partnerships with organizations that share our community-oriented values. Addgenies...Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey...

...tethered ligands hold the ligand in close proximity to the receptor, when exposed to light, the ligand engages...photoswitchable tethered ligands (PTL), and photoswitchable, orthogonal, remotely-tethered ligands (PORTLs). OptoXRs... them. PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody..., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K. (2019). Cortical layer–specific...

...affinity for the ligand, especially if the modified amines are located near the ligand binding site. A ...unbound material to calculate the amount of bound ligand (after subtracting out non-specific binding). Historically...dimers, may influence binding kinetics of the target ligand. Another important consideration is whether the...the affinity of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-...measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives have four...

...serve as slaves.  Meganucleases In 1989 the first ever DNA-editing enzymes, meganucleases, were discovered...capability of transforming the genetic code of living organisms and its societal implications even before the ...pneumoniae bacteria could be transformed from one organism to the other. This is now famed as the Griffith...genetics and SciFi.  Scientists around the world began looking for the magic molecule that serves as the...in 1990, just a year after the discovery of meganucleases. The storyline involves the resurrection of ...of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas) is that they ...

...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...plants Once you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones... of APT and any gene target of interest, only organisms that are actively silencing to a sufficient degree...

... post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...

... bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects...

... C. elegans, but activation of Raf-MEK-ERK signaling in the hypodermis and gut cells of C. elegans causes... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases...utility of  photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection...Zhou et al also used a photoswitchable CDK5 in C. elegans. See figures 4B and C in Zhou et al for details..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...

...synaptic proteins Fluorescent protein system for C. elegans Fluorescent timers for studying cell cycle length...fluorescent proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due...locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since it requires ...use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet...they fuse and fluoresce. The team engineered C. elegans cell lines to express wrmScarlet1-10 unattached...

...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...