...Where can I find information about using the AdEasy™ System? The laboratory of Bert Vogelstein has published...linearized construct, and virus can be harvested about 7-10 days later. Vogelstein designed multiple shuttle... Return to Top Frequently Asked Questions (FAQ) about Adenoviral Vectors Are adenovirus and AAV different...E2a and VA for replication. For more information about AAV, read our AAV guide . Can I make a stable cell...

...human genome with restriction enzymes would yield about two million DNA fragments, which is far too many...the molecular biologist’s toolbox. To learn more about different types of cloning methods check out our...restriction enzyme digestion. For more information about restriction enzymes check out NEB's website . Insert...endogenous expression levels). For more information about promoters, both bacterial and eukaryotic, as well...can be replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term...codon. Below you will find helpful resource tables about the genetic code. This table includes the nucleotide...

...biology, laboratory research, and career development. eBooks Download any of our eBooks to learn about popular...Playlists include: Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular...Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction enzymes...

...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...you about AAV components, serotypes, pseudotyping, and more....non-homologous sites in the genome, and do so at about a 0.1% frequency. Nonetheless, the majority of rAAV...enhanced brain uptake. AAV Variants As mentioned above, AAV improvements have included the production of...

...packaging methods. The three components described above (envelope, packaging, and transfer) are supplied... Return to Top Frequently Asked Questions (FAQ) about γ-Retroviral Plasmids How do engineered γ-retroviruses...may be used interchangeably. For more information about lentivirus, please read our lentivirus guide. Are...

...and are discussed in more detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA...Nme2Cas9 (from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than...targeting range for plant genome engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity...convert one base to another within a target locus of about 8 bp, specified by a gRNA. There are two main types...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...

...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable...when carrying out CRISPR experiments. Read more about how to design your gRNA . Browse Plasmids: gRNAs...to optimize your delivery conditions. Learn more about CRISPR delivery in mammalian systems . Validate ...

...the following guides to help you start learning about various scientific concepts related to our plasmid...Viral Vectors Find educational guides to learn more about the different types of viruses that are commonly...

... to conduct a pooled library screen. The figure above is an outline of how a gRNA library is used. Below...simultaneously in a single reaction. As discussed above, access to this technology is crucial for conducting...

...of interest is expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

...keep spreading the word about materials sharing, but we don’t have to worry about getting support from depositors...of course always being vigilant about resources, I’m not worried about the stewardship of this in the ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...on this. Addgene’s success is about the Addgenies. I never worry about their dedication, excellent intentions...more about Addgene Resources on the Addgene website Find plasmids for your research  Learn about our ...a lot of things we actually don’t have to worry about and that is something to recognize! Distributing...the last couple of years, we don’t have to worry about having enough space! There’s enough space here at...

...interesting content Talk about something you know a lot about and that you care about. Don’t try to present...engaging and memorable. If you read my story above about the presentation faux pas, you are more likely...the room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs ...dynamite presentation?  To that end, let’s chat about giving science talks and how to make them serve ...other people give great talks We are not talking about lab meeting here. Lifeless presentation of experimental...You want to give the audience something to think about, argue with, or learn from. Some of the best talks...topics many times, but I always seek out information about the particular audience and venue before tailoring...

...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...professors will not only talk about their own interests, but will ask you questions about your research goals and...Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...this:   However, as simply as it seems laid out above, there are many places where the PhD application...-hand, you can quickly reference your questions about the PI’s research or the name of the award you won...new way to allow you to get important information about the campus even if you can’t visit. By talking with...department, the lab setting, and how students truly feel about the program and faculty. Here are some questions...

... because we are highly collaborative, we love teamwork. Working collaboratively allows our team to best...clarification and again we collaborate with the Product Team who in turn collaborates with stakeholders to ...Scrum framework is based on an iterative and collaborative approach to development. Scrum teams plan and...that it provides transparency to everyone involved about the status of each project. This creates accountability... amongst the team. Whether we are a highly collaborative team because we are using the Scrum framework...These initial stories are usually a result of collaborations between Addgene’s Product Team and the stakeholders...Once we begin work on a story, we continue to collaborate closely with each other and with other teams....

...dig deep Talk to your professors about their research, and learn about what makes them excited to do what...sciences), people conduct research to answer questions about how the world works. As scientists, we probe beyond...curious person. I like to ask questions and think about the best way to answer those questions. I’ve met...enjoy, is worthwhile and can help us make decisions about what we want to do in the future. How do I get started...sense of what kind of research you would be excited about. I know quite a few friends who opted to join labs... a lot out there worth exploring! Being excited about the science allows you to ask more interesting questions...as graduate students (your teaching assistants), about their experiences. Research requires dedication ...

...matters Yes, yes it’s all about the science but really it’s not. It’s about the atmosphere that will allow...and are nervous about discussing data. There’s hesitation before answering questions about lab culture. ... emotions and assumptions. I’m not just talking about simple things like “Which route should I take to... and not very communal. That didn’t suit my collaborative learning style at all. Finally I rotated in ... did). Some of the information you will need is about you. It’s worth taking time to honestly evaluate...lab members can give open, revealing information about the culture and lab head. While it’s good to talk...same department as well. What do they have to say about this lab’s reputation? If you are choosing a training...

...learn about how researchers are using “big data” to look at Lyme Disease in new ways. Or learn about the...ot­her disciplines, are even less likely to read about our work. We therefore must discover new ways to.... Only on occasion do we then have time to read about research from other disciplines, or from other countries...communication through video     Video abstracts about research, especially published research, have grown...researchers have Facebook pages, few share any details about their research itself. Pinterest is also a favorite...created a new platform for sharing video abstracts about research: www.WeShareScience.org. WeShareScience...platform for sharing short, 3 to 5 minute videos about research. Videos can be shared by research scientists...

...possessed. Think about your shining moments. Recall a few times you really felt great about your work. Maybe...having a life outside my job. The same can be said about teamwork, communication, writing, management, and...you possess because any potential employers, collaborators, or co-workers want to know that you are a well-rounded...add value, not just “do the job”. When they ask about your creativity, your potential manager wants to...outside the box to solve a problem. When they ask about your communication style, your potential teammates...they have developed as part of a successful collaboration and fail to identify it as a personal strength...yourself in situations where you can learn more about possible strengths and weaknesses, and view everything...

...scientist anymore. Being a scientist is not about what you do--it’s about how you think. I have not worked at ...10 years, but when people ask me about my job, I have no qualms about saying “I’m a scientist.”    What...installment of the Addgene career guide, we’ll think about what causes this culture shock and how to overcome...counterpoints to some of the causes of culture shock above, but after polling some Addgene colleagues, we’ve...This is probably because you don't have to worry about experiments taking longer than expected or dealing... hour days at the lab, I encourage you to think about how much of that time you’re actually spending doing...candidates over the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is ...