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We narrowed to 642 results for: Emb;

Showing: 351 - 400 of 642 results

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T...AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...

  11. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...

  12. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...

  13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms... (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29...non-specific labeling. For labeling the plasma membrane, they tested different target sequences for improved...

  16. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... thereafter, using mouse instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al...A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://...

  17. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...interested in working for this lab and/or company. Remember to proofread your cover letter, and if possible... originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...for Excellence in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of...for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate. Her research focuses...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...https://youtu.be/EHHhiawIdWM Saving salmon, one embryo at a time – A video by LeeAnne Thayer, a Ph.D. ...University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http...

  21. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...harboring this mutation are deficient in outer membrane protease VII, which reduces proteolysis of the...pLemo (CamR) Expression of toxic, insoluble, or membrane proteins  T7 Express   IPTG-inducible expression...

  22. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...cells.   GFP is big GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter...Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells." Science 296.5569 ...

  23. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...empathy, a willingness to listen, and an ability to remember the right stories at the right time. Like many...morefamousquotes.com A good listener absorbs and remembers and makes the teller feel heard. I am not always...

  24. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...recommendations.  As you optimize your protocol, always remember to include controls to show that your antibodies...sample).  When choosing a primary antibody, first, remember that antibodies recognize very specific 3-dimensional...

  25. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several... guest blogger! Daria Shcherbakova is a faculty member at Albert Einstein College of Medicine. Her expertise...

  28. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... Symposium 2015. I personally participated as a member of the Career Paths Panel on the second day of ...people from all backgrounds are truly included as members of their research communities and are treated equitably...

  30. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression systems...vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525. Find...

  31. A Control for All Seasons

    Type
    Blog Post
    ...and equal protein transfer from the gel to the membrane. A loading control is critical for data interpretation.... Always probe the loading control on the same membrane that the target protein was measured on.    Flow...

  32. Antibodies 101: Validation

    Type
    Blog Post
    ...Show suitability for an intended application - Remember, just because your antibody performs as expected...is just as helpful as knowing when it will. And remember, antibodies are like puppies. Just because an ...

  33. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for...

  36. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...commonly added to aid in protein purification. Just remember to remove the stop codon for C-terminal tags and...yeast expression vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - ...

  37. Luciferase Plasmid Collection

    Type
    Collection
    ...imaging and other applications. And see PalmGRET , a membrane-anchored version useful for labeling extracellular... Blog: Luminescence Content last reviewed: 26 September 2025 Do you have suggestions for other plasmids...

  38. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation cytosine...Tsang in August 2020 and by Emily P. Bentley in September 2025. References and Resources References Chen...

  39. Plan Your Experiment

    Type
    Collection
    ...read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template...can transfect at high efficiency, such as human embryonic kidney 293 (HEK293) cells. For more difficult ...

  40. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...via genetically encoded proteins. PhoCl is a new member of this class that works via a different mechanism...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. These exciting papers ...

  41. Genetic Code Expansion

    Type
    Collection
    ...concentration, and the cell lines used previously. Remember that the orthogonal pairs of aaRS and tRNA that...opens in a new window) Content last reviewed: 19 November 2025 Do you have suggestions for other plasmids...

  45. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...tagged with #DeckTheLab throughout the month of December are eligible to win a hot chocolate party that...

  49. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on the welcoming committee...
Showing: 351 - 400 of 642 results