...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN (1981) “Western...Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton ...

...resources Use our Plasmids 101 eBook to teach students about basic plasmid biology Use or CRISPR 101 eBook to ...

...Additional resources on the Addgene blog CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to...resources on Addgene.org CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development ...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Communications Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE...paper in mSphere Find the dCas9 and dCas9-Mxi1 plasmids If you have a new CRISPR tool you’ve recently...

...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...you can learn more about in this blog post: CRISPR 101: RNA Editing with Cas13. Finally, the development... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...Additional Resources on the Addgene Blog CRISPR 101: Homology-Directed Repair Learn about base editing...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...

...writing of two blog posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications...

...insect cells, they are most often expressed from a plasmid in bacteria (Ahmed et al., 2012). One challenge...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...CRISPR-Cas9. Cell 170:1224-1233.e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang ...Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, Pinilla-Redondo R...Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes...-Cas9. Cell 167:1829-1838.e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu J-J, Bray...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang...RESCUE. Read the paper in Science Find RESCUE plasmids If you have a new CRISPR tool you’ve recently...

...on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...assays in the lab (for a refresher see Antibodies 101: Introduction to Antibodies.) Unfortunately, for ...

...Visit the Fluorescent Protein Guide Find antibody plasmids for your experiments ...

...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...antibody-based protocols - but thankfully this is an Antibody 101 post, so we'll keep it simple. If you follow the ...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...vector sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli and human...expression. Read the paper in Nature Find the CasX plasmids Drug inducible transcriptional activation and... Acids Research Find the drug inducible Cas9 plasmids E. coli genome-wide inhibition library In the...

...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....

...Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101: Viral...Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook, out...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors... Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W. (2014)...

...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with...in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization of...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...

...Check out CRISPR Topic Page Find CRISPR Nickase Plasmids Browse the CRISPR Guide ...

...Additional resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment?...More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR Guide  ...CRISPR-Cas. Molecular Cell, 74(5): 936-950.e5 (2019). 10.1016/j.molcel.2019.03.014 Zetsche, Bernd, HGootenberg...CRISPR-Cas system. Cell, 163(3): 759-771 (2015). 10.1016/j.cell.2015.09.038 Harrington, B., Lucas, Burstein...