...in between a protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl...acrIIA1 Listeria monocytogenes  pRAU168  No  pJH372  No  acrIIA2 Listeria monocytogenes  pRAU171 Partially...  Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  pCSW21  Yes...quickly and easily generate zebrafish transgenic lines to study neurobiology and neuorodgenerative diseases...compatible cloning sites for easy cloning of your genes of interest. In order to improve the study of specific...induction of structural plasticity in single dendritic spines. The set of CyRFP constructs available at Addgene... used a combination of deliberate and random mutagenesis to isolate the best RFP. They targeted known ...

...are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR library may target...target the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR...

...Harvard Medical School Klaus Hahn , UNC Richard Hynes , MIT Rick Horwitz , University of Virginia Denise...

...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...

...antibodies are produced by transfecting mammalian cell lines and collecting the secreted monoclonal antibody ...

...expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection Screening Multiplexed...

...plasmids for transcriptional repression of target genes....

...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription...

...Stockholm Bacterial Expression Plasmids (empty backbones/controls): ID Plasmid GenBank Key Features 26092...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...

...factors. gRNAs gRNA plasmids to target specific genes....

...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ...

...format include a variety of popular plasmids and backbones with high cloning potential. By making these plasmids...

...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...

...project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement...

...editing systems include targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty...

...extracellular protein interactions. Sun Y, Gallagher-Jones M, Barker C, Wright GJ. Analytical Biochem. 2012...