...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...that Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources...

...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin...lines generated from A549 cells transduced with lentiCas9-Blast 1 . A549 cells were transduced (MOI = 37...control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid...

...CRISPR imaging 51024 pSLQ1651-sgTelomere(F+E) Lentiviral vector with sgRNA targeting human telomeres 46913...dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that contains sgRNA targeting repetitive...

...Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base ...Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru 122871...

...2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the ...

...Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership...

...Addgene would be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - ...

...cell types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables...

...CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

... and requests are still pouring in! While our lentiviral service is going strong, the AAV service has ...

...et al., 2018). From this data, they generated lentiviral constructs using the High-Throughput Mutagenesis...

...mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown,...

...comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this represents the...

...CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis...

...unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used plasmids made...

...basic mammalian expression system and go for a lentiviral or a retroviral backbone. This is considered ...

...specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9...

...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...