...experimental hurdles I would encounter. This was invaluable to me as a bench scientist working at an institution...available. Even more valuable, however, was when she invited me to lunch for a “tweet-up” with other folks at...not participate but provided moral support was invaluable.  Mixing metaphors and scientists - Culturing...participants feel like their mentors weren’t as invested in how their meetings went. While this was expressed...

...months, in fact. In the Lee-Parsons lab, we are investigating how the production of cancer therapeutic drugs...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...transient methods are usually the first choice for investigating gene function, as these experiments can be done...species This will save you a lot of time. Don't re-invent the wheel. Test different leaf ages Usually younger...

...high-quality maps and annotations without us having to invest too much of our internal software development resources...zone As part of my position at Addgene I am now involved in Strategic Partnership Management, a very interesting...for discussions and potential collaborations. We invited experts from other universities and maintained ...ways to maintain a relationship - many of them involve personal meetings and common interest so naturally...

...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...

...experiment-based investigation of a hypothesis: proper controls! For every experiment, an investigator needs a ...an experiment lacking the proper controls are invariably inconclusive and unreliable. Proper controls ...tenet of responsible scientific inquiry and investigation. References: 1. The problem of pseudoreplication...

...hippocampus and the prefrontal cortex brain regions is involved in the formation of episodic memory, a special...for detailed mapping of the neuronal circuitry involved in learning and memory (Kohara et al., 2014; plasmids...reach the affected part of the brain in a less invasive manner . For example, in neuromuscular diseases...direct intracerebral application, which is more invasive and has a much higher risk of complications. ...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...Integrins are essential cell surface receptors involved in complex signaling pathways and the linkage ...researchers can measure this conformational change to investigate the role of specific integrins in cellular processes...subunit. Using these affinity reagents, the investigators inhibited other RGD-binding integrins, individually...for cell adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 ...

... biology. This type of cell labeling technique involves the introduction of a transgene construct containing...is Brainbow 2.0, which uses Cre to trigger the inversion of two genes aligned head-to-head. Another variation...variation, Brainbow 2.1, uses Cre to trigger the inversion of two tandem sets of genes aligned head-to-head...Mouse Model of Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): ...

...2.1 cleverly combines Cre-mediated deletion and inversion. The Brainbow-2.1 construct can express one of... or M-CFP.) The construct contains two tandem invertible segments, each with two fluorescent protein coding...color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre is removed...recombination is required to prevent constant inversion of the construct. Sanes and Lichtman used pronuclear...

... scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of... off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes...those sites, especially when DNA mismatches are involved. Two other approaches have been used - GUIDE-Seq...off-targets for non-cutters If your Cas experiment involves activation/repression or any other effect which...

...reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...transgene is no longer able to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While...opposite orientation (direction of arrows) the inverted transgene is reverted into the sense orientation...

...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...prep media, etc. "Research assistants" are more involved in the science, experimental design, and execution...genuinely excites them, and reach out to the PI to get involved. It not only demonstrates initiative and drive...

...testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This... the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting the...Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist named...which goes horribly wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting...

...for). For example, if you study neural pathways involved in addiction (such as the lab I work in), an obvious...constructs have been engineered with a double-floxed inverse open-reading frame (DIO). Upon coexpression with...mouse line or viral co-injection), the opsin is inverted into the correct direction and expressed (under...References 1. Tye KM, Deisseroth K. Optogenetic investigation of neural circuits underlying brain disease ...

... complex DNA constructs, particular for those involving recombination elements such as FRT or Lox sites...of sequences in bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs...molecules, allowing labs to precisely and non-invasively control neuronal signaling. For more information...recognition sites that flank a DNA module in an inverse orientation, so that multiple DNA components can...uses two plasmids in tandem, with Cre-dependent inversion determining between 0 and 2 FPs expressed from...

...greater scientific field, potential customers, investors, or other relevant entities or individuals. A ...one that you can pay for, but one that you are invited to join (selected due to expertise in field). Publicity...it as documentary evidence for your case – the invitation to be a peer reviewer needs to be addressed to...diagnostics, healthcare IT, boutique consulting, investment banking and venture capital areas.  We place ...

...I pay the invoice? After your Addgene order ships from our facility, we will send an invoice to the billing... address that you listed during checkout. This invoice will reference your Addgene order number and PO...

... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...help scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied...

...virologists who don't have the resources to get directly involved with COVID-19 to researchers in other disciplines...resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...

... S et al., 2002) and many downstream complexes involved, it can be difficult for scientists to understand... be activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer.... Lastly, the activity of both antagonists and inverse-agonists could be measured using the TRUPATH system...