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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 538 results for: invs

Showing: 121 - 140 of 538 results

  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...experiment-based investigation of a hypothesis: proper controls! For every experiment, an investigator needs a ...an experiment lacking the proper controls are invariably inconclusive and unreliable. Proper controls ...tenet of responsible scientific inquiry and investigation. References: 1. The problem of pseudoreplication...

  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... biology. This type of cell labeling technique involves the introduction of a transgene construct containing...is Brainbow 2.0, which uses Cre to trigger the inversion of two genes aligned head-to-head. Another variation...variation, Brainbow 2.1, uses Cre to trigger the inversion of two tandem sets of genes aligned head-to-head...Mouse Model of Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): ...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Integrins are essential cell surface receptors involved in complex signaling pathways and the linkage ...researchers can measure this conformational change to investigate the role of specific integrins in cellular processes...subunit. Using these affinity reagents, the investigators inhibited other RGD-binding integrins, individually...for cell adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 ...

  4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...2.1 cleverly combines Cre-mediated deletion and inversion. The Brainbow-2.1 construct can express one of... or M-CFP.) The construct contains two tandem invertible segments, each with two fluorescent protein coding...color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre is removed...recombination is required to prevent constant inversion of the construct. Sanes and Lichtman used pronuclear...

  5. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of... off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes...those sites, especially when DNA mismatches are involved. Two other approaches have been used - GUIDE-Seq...off-targets for non-cutters If your Cas experiment involves activation/repression or any other effect which...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...transgene is no longer able to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While...opposite orientation (direction of arrows) the inverted transgene is reverted into the sense orientation...

  7. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...prep media, etc. "Research assistants" are more involved in the science, experimental design, and execution...genuinely excites them, and reach out to the PI to get involved. It not only demonstrates initiative and drive...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This... the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting the...Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist named...which goes horribly wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...for). For example, if you study neural pathways involved in addiction (such as the lab I work in), an obvious...constructs have been engineered with a double-floxed inverse open-reading frame (DIO). Upon coexpression with...mouse line or viral co-injection), the opsin is inverted into the correct direction and expressed (under...References 1. Tye KM, Deisseroth K. Optogenetic investigation of neural circuits underlying brain disease ...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... complex DNA constructs, particular for those involving recombination elements such as FRT or Lox sites...of sequences in bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs...molecules, allowing labs to precisely and non-invasively control neuronal signaling. For more information...recognition sites that flank a DNA module in an inverse orientation, so that multiple DNA components can...uses two plasmids in tandem, with Cre-dependent inversion determining between 0 and 2 FPs expressed from...

  11. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...greater scientific field, potential customers, investors, or other relevant entities or individuals. A ...one that you can pay for, but one that you are invited to join (selected due to expertise in field). Publicity...it as documentary evidence for your case – the invitation to be a peer reviewer needs to be addressed to...diagnostics, healthcare IT, boutique consulting, investment banking and venture capital areas.  We place ...

  12. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...I pay the invoice? After your Addgene order ships from our facility, we will send an invoice to the billing... address that you listed during checkout. This invoice will reference your Addgene order number and PO...

  13. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...help scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied...

  14. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...scientists around the world to conduct robust investigations into the human transcriptome. The MORF library...feels both welcomed and resourced for success. I invite you to join me here, and on my LinkedIn page, as...perspective on Addgene through her LinkedIn page. She invites you to connect with her there. More on the Addgene...

  15. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...virologists who don't have the resources to get directly involved with COVID-19 to researchers in other disciplines...resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...

  16. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ... dissection video. I did that in January. This involves going in front of a camera and kind of walking...operations departments at eScience Labs. This can involve traveling with the sales team to meet faculty and... of determining if projects are feasible. This involves thinking about kit cost and safety.  Being a manager...

  17. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...for in vivo behavioral studies because it is non-invasive and offers a high degree of control over time ...larvae of zebrafish are translucent and allow for noninvasive live imaging with fluorescent tags and activation...Wyart et al., 2009). The CPG is a neural circuit involved in locomotion that generates periodic motor commands...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... S et al., 2002) and many downstream complexes involved, it can be difficult for scientists to understand... be activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer.... Lastly, the activity of both antagonists and inverse-agonists could be measured using the TRUPATH system...

  19. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...fairground rides and other fun activities and even invite members of the public to attend. We thought we’...level academic debates for its members to become involved with. The society provides talks and career support... Advice from Addgene Bloggers Learn How to Get Involved in a Profession Organization ...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...key parts of an AAV plasmid. Inverted terminal repeat (ITR) sequence Inverted terminal repeats (ITRs) are...Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Human...
Showing: 121 - 140 of 538 results