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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 241 results for: grna

Showing: 121 - 140 of 241 results

  1. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... in C. elegans. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  2. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... other insects. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...

  6. CRISPR Plasmids - Plants

    Type
    Collection
    ... use in plants. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...for use in yeast. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...use in bacteria. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  9. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of ...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  10. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...expression systems. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  11. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  12. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the non-targeted...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  13. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...Cas9. With many PAMs available, a large number of gRNAs could be screened for the most efficient editing...different mutations in different patients. While these gRNAs would still need to be optimized and assessed for...the best when paired with the highest-efficiency sgRNA. The PAM sequence near this site is AGC, so in a...

  14. CRISPR Plasmids and Resources

    Type
    Collection
    ...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723...

  17. Editor's Choice, August 2016

    Type
    Blog Post
    ...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...

  18. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...lab. The Bikard library contains ~93,000 unique gRNAs that target random positions along the E. coli MG1655...targeting requires two sgRNAS: one that targets the knock-in site and a universal sgRNA that targets the donor... the genomic loci of interest. As the universal sgRNA is not predicted to target sites in the human, pig...

  19. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...transcription. This interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  20. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...spacer and the remaining sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the ...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA... tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8...complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “... on the 3’ end of an RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this ...
Showing: 121 - 140 of 241 results