...guidance. Every plasmid shared, every collaboration formed, and every breakthrough enabled brings us closer...data for over 150,000 plasmids, empowering bioinformaticians, data scientists, and developers to unlock...Schofield, C., … Laflamme, C. (2024). A consensus platform for antibody characterization. Nature Protocols...

...base pairs to the repeat section of the crRNA, forming the repeat:antirepeat duplex. tracrRNAs also contain...tracrRNA sequences in a “single-guide RNA” that performs both functions. Cas9 cuts both the target and ...

...While in Grad School How to Lead a Great Meeting Form Your Own Peer Mentoring Group...interdisciplinary seminars, such as those in bioinformatics or chemistry, even if your primary focus lies...

... collection by filling out our Suggest a Plasmid Form. We look forward to helping microbiologists from...sharing and was also a great place to catch up with former colleagues! I ran into past ASM President Tim Donohue...

...technologies on our blog and encourage you to fill out this form if you’re ever interested in writing your own blog... upon you, our depositors, giving us as much information as possible during the deposit process. Special...

...to the biology and chemistry societies that will form the core of their scientific networks in their many...society also organises PhD information events and summer internship information sessions to help undergraduate...the answer is probably no, but these fun and informative events are one of the main ways budding young...intro sessions, and give out useful items and information to students. Freshers fairs are also where many...

...cationic liposome) or electrical (electric current forming membrane pores) transfection. These techniques ...sequence to be placed on the packaging plasmid forming a vector that could create replication competent... See the NIH’s biosafety guidelines for more information and always be sure to consult your institution...transduction for instance. I hope you will find this information useful for your experiments. Don’t hesitate to...check our lentiviral vector guide to get more information and to get access to additional protocols and...

...antibodies are produced from hybridomas. Hybridomas are formed by fusing B cells that have been extracted from... of the heavy and light chains fused together to form a single protein that can recognize the target protein...sequence of amino acids, or only in their 3D conformation. Types of antibodies and production In the immune...Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...

...Internal Revenue Code 501(c)(3). That means we were formed to benefit the public, not private interests. On...improving access to useful research materials and information.” Addgene is here to help scientists accomplish...the world to gain access to new materials and information. Core values drive decisions at Addgene If you...customer service that is consistently timely, informative, friendly, and professional Addgene is a thought...

...for the Addgene Blog? Fill out our guest blogger form to get started!   Thanks for all your hard work!...post from Keith Pardee and Alexander Green (both formerly at the Wyss Institute) to learn how these amazing...Microscopic Tools In this post, Jessica Polka, formerly a postdoctoral researcher in the Silver Lab at...set of DNA sequences. In this post, Alan Wong (formerly of the Lu Lab at MIT) shows how you can use CombiGEM...

...additional plasmids with the Viral Service Suggestion Form. Lentivirus As some of the most popular lentiviral... gene, replace it, modulate its expression, or perform a genome-wide screen, we’ve got viruses to help...vector pages as well as expanded virus protocols, information on how we produce and test virus, and quality...

...) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when transcribed which aids...

...please let us know by filling out this suggestion form. As we build the collection, we hope that you will...the Trimmer Lab. Information about the antibody, the target antigen, and performance in various applications...The transparency begins with sharing sequence information for the antibodies in our catalog, so that you...Addgene’s blog and Antibody Guide, which include information about immunofluorescence, conjugates, isotypes...share plasmids with each other. Together, we can transform the antibody sharing landscape as well.     References...

...signal to visualize low-abundance targets.  Pre-formed primary and secondary antibody complexes (Figure...

...researchers were already on a quest for smaller forms of antibodies and had begun developing Fab fragments...

...can also find several other constructs in plasmid form. Fig. 1: Summary of the approach and outcomes...-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions...experiments with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective...engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova...

...growing effort to address this obstacle in its many forms. A short stroll from my office to the tearoom would... out a comprehensive review of the literature informing the notion of “heading” in soccer as a possible...and precautionary regulation was all necessary to inform the experimental aspect of my thesis: a survey ...science communication, although the detritus of misinformation in the wake of a global pandemic can make for... how far we have yet to go. In a world where information becomes easier to access by the day, it has never... how we receive, process and respond to that information. Alex Gilbert is a PhD student at the University...

...seven patients diagnosed at their hospital with one form of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee...desire to understand the early events in the transformation of healthy cells into melanoma cells. They’...

...relationships, so that you can reap rewards in the form of reaching career development goals. Check out ...additional resources can be downloaded in E-Book format at the end of this post. If you have been following...most of this new relationship? Consider adding formality and active goal setting to your mentoring relationships...out Joanne's Reddit AMA Add a little formality to mentoring Just getting together and chatting with another...phone, etc.)? Make agendas for your meetings, take informal minutes on what was covered and learned, especially...

...fresh stocks. Most antibiotics are stable in powder form, but quickly breakdown in solution. Storing aliquots...how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient process...