...Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre....Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...

...with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus ...activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 ...infection period rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR...

.../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* Wilson 105558 pENN.AAV.CamKII...site-specific recombinases, which can be used to control gene expression. Dre AAV ID Name Promoter Fluorophore...2, 5, 8, 9, rh10 Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre...EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB Wilson...

...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1...mTurquoise2 Brightness standard used as positive control to characterize mTurquoise2 pmTurquoise2-T2A-Venus...T2A-Venus(L68V) Brightness standard used a negative control (no FRET) with pmVenus(L68V)-mTurquoise2 Back to...to Amber via a 32 amino acid linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino...

...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...cannot be released, adding temporal and/or spatial control to the system. pSQT1313 from the Joung lab allows...

...modifications in the same strain. MazF expression is under control of the AOX1 promoter whose expression is induced...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...

... laboratories to carry our independent quality control for a range of testing, from animal doping to forensics...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...

...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted...

...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...

...pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F...production , these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and...

...hUbC-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA ...shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression...expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway...expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression...other versions of pULTRA. Moore 19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895...plasmids. Elledge 21373 pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid...14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1 selection. ...

...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...find antibody plasmids for: Common antigens such as GFP or mCherry Monoclonals, Nanobodies, Sybodies, or ...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their...

...overcome by using a Tet-inducible virus to allow for controlled expression of reprogramming factors. Check out...the presence of transgenes is still an important control. Additionally, Sendai virus doesn’t rely on promoters...that multiple transfection makes it difficult to control the dose of plasmid the cells receive over the ...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression Mediated by a Sendai Virus-Based...

...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...metabolites, leading to conformational changes that control the expression of coding RNA. Spinach-based riboswitches...

...injury markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs...

...research utilize light to detect, measure, and control molecular signals and cells in order to understand... genetically-encoded tools for light-activated control of proteins; e.g., microbial opsins and optical...subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and off the light...illuminated. There are several different ways to control opsin expression. Generally, the most robust and...-ChR2 mouse, where ChR2 is expressed under the control of the vesicular gamma aminobutyric acid (GABA)...GABAergic neurons being activated by the light is controlled via the placement of the optic fiber. Using viral...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins offer...

...Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You...to bind to an unallocated codon. Therefore many controls must be used to make sure that these conditions...Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli various unnatural...or DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...C321.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter ...reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, ClpS...

...26973 AAVrg pAAV-hSyn-hChR2(H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret...inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics...more efficient tools for light mediated neuronal control and we are expanding our viral service to make ...own experiments. To this end, we offer specific control AAV in smaller aliquots of 20 ul for serotype testing... service has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing...response to action potentials, is now offered under control of the CAG promoter for ubiquitous expression. ... 44362  AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Controls Plasmid Serotype Name 50459 AAV1 pAAV-hSyn-DIO-mCherry...

...pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New ...pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls Deisseroth New... pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB Controls...Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG...