..., Word docs, and the like is likely stored in online file storage tools like Dropbox, Box, One Drive, ...commonly used online file storage tools, as illustrated in this video, and to data stored on institutional and...like image banks and sequencing files is likely stored in institutional and lab servers specifically designed... conveniently reference specific pieces of data stored externally in the record of research compiled in...fully supported API that enables development of custom integrations with other tools and resources. As... in the documents to data in the external file storage resources remain live. This means that someone ...research and commercialization community in greater Boston, while at the same time keeping one foot on the...

...human cryostat tissue sections. J Histochem Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/...Fighting with antibodies to produce immunohistochemistry images that are crisp, bright, and lacking in...avoiding background secondary binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your...Randolph-Habecker J (2013). A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free...Immunofluorescence, and Multiple Antibody Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620...Monoclonal Antibody to Mouse Tissue Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813...Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding...

... it out. 4:00 PM--Respond to customer help tickets. Catch up on customer help tickets, and respond when...advocate for the customer and help build software and technology tools with the customer in mind. You can...Oxford Nanopore Technologies, I work with our customers in the US to learn where the gaps are in their... so many different people! I communicate with customers, software developers, as well as team members ... It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’t wait to ...know what to work on next to help alleviate any customer pain points. Many thanks to our guest blogger...

...usual quality and success. Customer and technical support Our customer and technical service brands...helpful and the customer and technical service teams put 100% effort into helping our customers every day in...have for the future. At Addgene we will never ever stop planning, perfecting, and learning, but there are...scientific fields.” Thanks to our legal team, and great customer service, somehow we have scientists actually loving...offer online for Addgenies and to serve external customers. I also can’t wait to see where our new data initiatives...to do it quickly. Hiring is hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive...

... to learn things quickly. Story telling. The ability to know what’s a story and how to hook your readers...feels that storytelling is an impactful tool for science communicators. “The power of a story is to make...there’s always plenty of writing: plain-language stories about Fred Hutch research, pieces for donors such...that can be a very painful process. And certain stories can make you feel like ‘I’m a terrible writer. ...through connections and that’s something that good storytellers in science can do.” Susan’s number one piece...social media as a scientist here Learn more about storytelling in science ...

...presence of a stop cassette upstream of the target gene in the targeting vector. The stop cassette is floxed...embryonic stem cells that are then injected into a blastocyst. There are several types of genetically engineered...Modified embryonic stem cells are injected into a blastocyst which is then implanted into a female animal ...embryonic stem cells, which are then injected into a blastocyst. Nucleases can also be used to engineer knockout...create mosaic animals where not all cells in the blastocyst were successfully edited by the injection of ... variations of this technology can be used to customize the effects of this recombination. To generate...floxed, so Cre-mediated recombination deletes the stop cassette, which permits expression of the target...

...bring out these interesting stories and remind the panelists of the stories or topics you know they can... taking good notes and discovering interesting stories, riffs or opinions that each panelist can contribute...identify the funny, provocative or interesting stories that will make for a fun discussion. People are...they can see each other) on comfortable chairs (stools if you have to, but this is tough for women in ...especially short people – which I am, so I hate stools). I like it when the moderator sits in the middle...question in a way that elicits those interesting stories and opinions. Don't let all the panelists answer...moderator's job to politely but firmly get them to stop talking and redirect the conversation. The person...

...production is Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins...applications for validation include western blot, immunohistochemistry, and immunocytochemistry. It’s important...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ... to their parental hybridomas. Aggregation and storage Aggregation of antibodies may impact their quality...testing. An aliquot of our recombinant antibodies is stored at 37 °C for a period of two weeks. After this ...that are not equally effective are shipped to customers on ice, preventing any temperature shifts. Of ...we are also cautious during the aliquoting and storage process to prevent dropped tubes. Now that your...

...primary role is customer support, but there are eight other teams who still retain a customer support element...when I discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...because confirming that every person has read and understood every communication that gets sent out through...other responsibilities. Knowing which aspects of customer support each team “owns” is critical for day-to-day...-to-day operations and keeping our customers happy, but inevitably there will be gray areas that either...another job, it will be important to retain the stories and examples that have guided your resolution processes...

...either side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed...of cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck...step, the effector is expressed. In cells where the stop cassette remains, the effector is not expressed ...competing attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis...attP site. Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...preferentially leads to gene constructs where the stop cassette remains following recombination. This strategy...

...would love to tell such a dramatic story, but it wouldn’t be my story. I love academics. If I didn’t, it...there was even a time when I was too nervous to ask store clerks for help at the mall. In undergrad I was ...to open up a little after I dropped myself into Boston University with none of my old friends to fall ...thought that I would be best poised to share the stories and difficulties behind great discoveries by tackling...actually finishing up this post on a plane back to Boston from Kentucky where I spent a couple of days talking... mission to make research easier. I’m hoping my story demonstrates that transitioning from academia doesn...

...fresh stocks. Most antibiotics are stable in powder form, but quickly breakdown in solution. Storing aliquots...them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...tool in molecular biology, yet scientists rarely stop to think about how much easier it makes our lives.... For instructions on how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics...antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt genes at the chromosomal...

...their CPP system to study protein transduction, D’Astolfo et al. made the surprising discovery that both...NDSB-201) were required for protein delivery. D’Astolfo et al. coined the term iTOP (induced transduction...that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series of primary cell...resulting in GFP expression. Image courtesy of Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP...Having tested iTOP using Cre-lox recombination, D’Astolfo et al. were eager to see if iTOP would be compatible...cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances, the potential...Efficient Intracellular Delivery of Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers ...

...service. Some AAV1 tools are available through our in-stock viral prep service.  AAV2 Despite being isolated...service. Some AAV2 tools are available through our in-stock viral prep service.  AAV5 AAV5 is the most genetically...service. Some AAV5 tools are available through our in-stock viral prep service.  AAV8 AAV8 has high transduction...as plasmids in the repository, as part of our in-stock viral prep service and can be selected as a serotype...service. Some AAV8 tools are available through our in-stock viral prep service.  AAV9 AAV9 has relatively broad...service, but some AAV9 tools are available as in-stock preparations. AAV retrograde (AAVrg, also known ...  Some AAVrg tools are available through our in-stock viral prep service.  In summary Your choice of ...

... maintain many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington...start of the day and throughout daytime. They mostly stop emerging at night. An incubator with a light cycle...genetic crosses. More importantly, this knowledge will stop you from embarking on a fruitless journey of trying...information about gene function, while others offer fly stocks and other reagents. If you’re ever confused about...where the gene is expressed within the fly, whether stocks of mutant flies exist, whether any phenotypes have...mentioned the gene. The Bloomington Drosophila Stock Center at Indiana University is another tremendous...

...anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources. As editor...picked our viral vector service as one of those stories. Viral vectors are viruses engineered to be safely...available and number of requests tell only part of the story. Viral vectors are, after all, a delivery method...blog, you may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the ...primary literature, soliciting information from requestors and depositors, and identifying trends in our...actually show you if the vector was useful for the requestors or how it performed. Addgene launched our AAV...platform for the sharing of that exact data. Here, requestors can upload their experimental data in a standardized...

...norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image...are five more model organisms that have left a historical impact on the scientific community. (And, check...  The E. coli genome has an extensive research history and characterization. Many proteins and processes...organisms in the world. With its extensive scientific history, highly characterized genome, and widespread utilization...organisms is its thoroughly developed and defined history in research (Krämer, 2015). As a result, a vast... https://doi.org/10.1159/000490898 Gibbs, R., Weinstock, G., Metzker, M. et al. Genome sequence of the...

...copies of a tRNA that specifically recognizes amber stop codons (TGA) to encode phosphoserine, the Sep-tRNA... the phosphorserine OTS recognizes the TAG amber stop codon, using the OTS in an unmodified bacterial ...would result in read-through translation of many stop codons and detrimental effects on cell growth. The...was genomically recoded to eliminate all 321 amber stop codons (TAG), replacing them with TAA (7). This ...TAG codon should encode phosphoserine instead of a stop codon, which will allow full length GFP to be translated...can also be cloned with an ORF of interest for customized experiments. Once the ORF of interest is cloned...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated...

...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

... chose the stop motion format for our videos as it is the most approachable for LEGOs. Stop motion consists...pictures together to form the full movement. Producing stop motion videos is very time consuming; producing ...toys” gaining life and working as scientists in a custom made, colorful laboratory. When we started, the...child to work. If you have an odd or quirky lab story you’d like to see portrayed in LEGO form, feel free...us at [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest...