...parasite, P. falciparum . Please see the following reference for more information: A Human Platelet Receptor...

... Addgene’s TAL effector and TALEN kits here . References Efficient design and assembly of custom TALEN...

...fused between ECFP and Venus Back to Top FRET Reference Standards The following plasmids have well-characterized...

...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...

...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...

...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrh10...

...Their ability to self-renew or replicate while maintaining the undifferentiated “stem” state and, Their...to self-renew is greatly diminished. Embryonic and adult stem cells can be used for different research...contributed by Kusumika (Kushi) Mukherjee, editor of Trends in Pharmacological Sciences, a Cell Press reviews...special types of cells that can develop or “differentiate” into more specialized cells with specific functions...function adequately [1]. Stem cells’ ability to differentiate into multiple cell types makes them useful models...Their potency or capability to differentiate into specialized cell types [2]. We general speak of 4 levels... cell has the ability to produce all of the differentiated cells in an organism, including the embryonic...

... 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

...molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium...

...comes the need for new conference room names. The Addgenie’s favorite conference room naming scheme? Gene...largest conference room at Addgene is named Sonic Hedgehog but this is not a direct reference to the video...community. Not surprising, this is where most of our conference room names originated from. Let’s take a look...Groucho, a transcriptional co-repressor The first conference room, that is actually used as a break room for...diffuses down a concentration gradient exerting a different effect on the developing embryo depending on its...required for spermatogenesis One of our smaller conference rooms is named Cookie Monster. cookie monster... bagpipe, two genes important for mesoderm differentiation Now, let's turn tinman and bagpipe, two genes...

...induces mutation at rates much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs...

...making Integrase deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and...

...made from a selected set of plasmids that are currently in our repository. AAV are distributed as 100 ...

...788679 Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng S, Ren M, Jiang S, Ni H, Zhou C, Gong H, Luo M (2018) Cell-type-specific...in decreased efficiency of recombination and preferentially leads to gene constructs where the stop cassette...likely generalizable to other systems as well. References: del Valle Rodríguez A, Didiano D, Desplan C...plasmids at Addgene Read our Molecular Biology Reference ...

...10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen... investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An... of the scientific research infrastructure. References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S,...(2006) Is there an ideal animal model for SARS? Trends in Microbiology 14:299–303 . https://doi.org/10.1016...

...10535 (2018). 1 10.1074/jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed...park. Happy editing!   Resources and References References Yu, Y., Guo, Y., Tian, Qigi, Lan, Y., et...overhangs at the break site – an early HR step (Moreno-Mateos, et al., Nat Com). Another method used ...the break, potentially shuttling repair down a different pathway (Charpentier et al., Nat Com).   ...Cas9 delivery. eLIFE. (2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez, J. P., Rouet, R., Vejnar...

...-023-02050-w Yi, Z., Zhang, X., Wei, X., Li, J., Ren, J., Zhang, X., Zhang, Y., Tang, H., Chang, X., Yu...) enzymes. Various other proteins included in different CGBE tools allow the researcher to direct the ...component of the nucleoside inosine. Created with BioRender.com. Inosine excision leads to the first adenine...a translesion synthesis polymerase known to preferentially incorporate A bases across from abasic sites...indicate natural cellular processes. Created with BioRender.com. The first deaminase-free base editor allows... Figure 3: Mechanism of gGBE. Created with BioRender.com. Base excision editing extends to thymine...recognize thymine (T) or cytosine (C). Several different teams succeeded with this approach around the ...

...Educational Resources Molecular Biology Reference Molecular Biology Reference Jump To: Molecular Biology Basics...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing...verification Resources Genetic Code Webpage and Blog References Protocols Origins of Molecular Genetics The concept... Joshua Lederberg coined the term plasmid, in reference to any extrachromosomal heritable determinant....cells contain the same genetic information as the parental cell, and are thus termed clones of the original...molecular biologist’s toolbox. To learn more about different types of cloning methods check out our guide on...promoter (e.g., a liver-specific promoter). The strength of the promoter is also important for controlling...

...If possible, include an AAV reference sample of known titer. The reference material should have a titer...an internal reference virus that is 1 x 10 13 GC/mL. We recommend always using a reference within 1-log...should observe differences in Ct values that make sense for your dilutions (~3.3 difference Ct for a 10-...This protocol was validated using an internal reference AAV of known titer, 100837-AAV1, and by measuring...master mix should also contain an internal passive reference (typically ROX dye), to normalize non-PCR–related...using this plasmid, but will change if you use a different plasmid. Sample Calculation Size in bp of Addgene...standard dilution. Dilute DNase-treated and AAV reference samples according to the dilution scheme in the...

... DMEM complete + 10 µg/mL polybrene by diluting 20 µL of 10 mg/mL polybrene into 20 mL media. Thaw the...with DMEM complete + 10 µg/mL polybrene, the final concentration of polybrene in each well should be 10 µg...glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 ...21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter, Nalgene... 5mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and FBS lots can promote or inhibit transfection...of the lentivirus in DMEM complete + 10 µg/mL polybrene. Note, this is just a sample of possible dilutions...Lentivirus (μL) Volume of DMEM complete + 10 µg/mL polybrene (µL) 0 0 500 1:5 300 200 1:10 150 350 1:50 30 ...