...the target gene’s native locus, but introducing large genetic changes in C. elegans with CRISPR can be...(ssODN). So how can you use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach...

...the Biomek FxP is it’s customizability. It has a large deck that can house many different types of devices... DNA purification We're developing ways to prep large quantities of DNA because some countries just can...be used for a mini prep, except it does it on a larger scale. Using the BIOROBOT Universal Platform is...

... immunoglobin component that can be produced in large “copies.” This homogeneity circumvents the batch-to-batch...reproducibility and cost-effectiveness for broad and large quantity uses. We therefore use both rAb and mAB...as non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and whole...

... PASTE, a drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing:...

...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during...body. In contrast, these dynamic processes are largely absent in adult organisms, where tissues structures...adapted to studying other systems in which there is large scale cellular movement throughout an organism. ...

...(~150 kDa) and Y-shaped because it contains two large heavy chains and two small light chains that stick...optimally binding sybody, you want to start out with a large and diverse pool of sybodies that hypothetically...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...

...injection site is straightforward, as it targets large muscle groups (usually the thigh), though it is ...theoretically be performed anywhere on the body, somewhere large, flat, and easily accessible (like the back) is ...

...population does not move even after applying very large compensation values (100+), consider what two markers...diagonal line in the top right corner of the plot and large compensation values will not move it (Figure 3)....

...already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the Cas9 gene...compared to other viral vectors, AdVs can provide a large transgene packaging capacity. For instance, with...

...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The...through insertional mutagenesis. These risks are largely based upon the vector system used and the transgene...

...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

...the University of Goettingen, and several other large research institutions. The following graphic depicts...institutional and lab servers specifically designed to hold large amounts of data. Although it may be useful to selectively...

...fusion proteins be in close proximity and present in large enough quantities (and the correct stoichiometric...nature of BiFC also makes it easier to generate a large number of constructs for high-throughput screening...

...novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running the marketing...challenging. For instance, the client might need to get a large amount of market research done to inform a go/no-go...

... fused to a dimerizing FP has potential to form large stable polymers of dimers. 3) FRET biosensors that...dimerizing FP is fused to the POI. These structures are large (microns), bright, non-physiological, and difficult...

...which is formed by a complex of two proteins: the large (L) catalytic protein and its cofactor VP35. VP30...necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid would be...

...plasmids in the library.  Spinfection If you have a large number of cells to transduce, you may want to consider...pooled libraries can be intimidating and require a large amount of experimental work, but with this guide...protocol. After functional titer is determined, larger lentiviral preps can be used for screening-scale...

...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression of lentiviral... vivo CRISPR screens. AAV vectors have played a large role in these advancements, due to their many benefits...of molecular pathways. These screens have gotten larger, easier, and more comprehensive thanks to the consistent...

...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved ...their own data, with some smaller labs feeling that larger, better resourced, groups would harvest and reanalyze...