...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...

...well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc...BBB via interactions with a similar protein called LY6C1. This protein is expressed in both BALB/cJ mice...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While... it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019; Huang et al...capsids that rely on interactions with those two proteins to cross the BBB are often not effective in NHPs...2023 AAV.CAP-Mac CNS: neurons (rats, infant green monkey, rhesus macaque); vasculature (adult marmosets...astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys, rhesus macaques N/A Chuapoco et al....

... and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant...Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination...

...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under the... the control of various promoters. Sensor Green Calcium Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8...

...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...exciting for scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling...

... BirA, has been used to identify and study protein-protein interactions in proximity labeling techniques...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...

...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...signaling protein that the receptors couple to: Gq-DREADDs signal through the Gαq/11 G-protein and activate...bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led...The first chemogenetic receptors were based on G-protein coupled receptors, or GPCRs. The largest class ...into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read the GPCRs: How Do They ...DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs, show insensitivity...employed. Gi-DREADDs signal through the Gαi/o G-protein and inhibit neuronal signaling by inhibiting adenylate...

...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Omi-mCherry HTRA2 mCherry LTR Parkinson's Douglas Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Erik Roberson 179896 pMKRQ_11_DExCon-mNeonGreen-R25 RAB25 mNeonGreen TRE3GS Parkinson's Patrick Caswell ...Parkinson's Randy Schekman 205713 mNeonGreen-alpha-synuclein SNCA mNeonGreen CMV Parkinson's Randy Schekman...