We narrowed to 258 results for: GFP
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TypeBlog Post...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...
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Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell... -
Plasmids 101: What is a plasmid?
TypeBlog Post...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome ... -
New Viral Vectors - Winter 2025
TypeBlog Post...serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep ...Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase James M. ...Recombinase James M. Wilson New serotype pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 AAVrg Recombinase James M... prep AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) AAV-PHPeB Optogenetics... prep AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) AAV-PHPeB Optogenetics... prep AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) AAV-PHPeB Optogenetics... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple... -
Antibodies 101: Affinity Tags
TypeBlog Post... (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can... -
Year of the Camelid: Antibody Style
TypeBlog Post...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation. sdAbs ... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...endocytosis of a fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post... University with David Goldstein’s lab, will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post..., hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following the expression pattern... allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently... -
Brain Armamentarium
TypeCollection...James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting...enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell James M. Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct...construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available...our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors... testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes. This...recently released these controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad...broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression...interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter...mCherry in the absence of Cre, and expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium ... -
Another Pathway into Cells: iTOP
TypeBlog Post...Neuronal stem cells transduced with iTOP resulting in GFP expression. Image courtesy of Diego D'Astolfo. ... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP S2TAG...Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306 C321...S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...