Quantifying DNA? Here are Five DNA Quantification Methods to Consider
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Blog Post
...you only need 1-2 ul of sample and the run time is just a few minutes per sample. During the run, DNA fragments...absorbance. Then you measure the absorbance of the DNA sample.
These absorbance measures give you an idea of...especially when you expect low concentrations in your samples and are often used to quantify DNA for next generation...fluorescence-based methods require a standard curve, a set of samples with a known DNA quantity and their corresponding...That way, you can compare the fluorescence of your sample against this curve to quantify your DNA prep. Though...yourself as many fluorometers will calculate your sample concentration for you.
Agarose gel electrophoresis...concentration of each band in the ladder. You then run samples of your DNA at different dilutions and quantify...