...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...feature some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation...past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These...s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent... about mNeonGreen, mTFP1, and mWasabi and find plasmids containing these fluorescent proteins at Addgene...graphics. PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection...

...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

...Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each HIS Own...

... Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...protein fusions Browse the fluorescent proteins plasmid kits ...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...Proceedings of the National Academy of Sciences 101.29 (2004): 10554-10559. PubMed PMID: 15247428. PubMed...

...resources on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...to share the latest edition of our Viral Vectors 101 eBook!  This practical guide to using viral vectors...

... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect...Addgene's Antibody Plasmid Collection Resources on the Addgene blog Antibodies 101: Reading a Flow Plot...Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

...on the Addgene blog Antibodies 101: Epitope availability Plasmids 101: Protein Tags Antibodies: Of Myc...

...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...

...Vectors 101: An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101: Systemic..., et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected as a serotype..., et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral...retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not...Request Browse all in-stock AAV preps Browse all AAV plasmids ...Clinical Development, 26, 331–342. https://doi.org/10.1016/j.omtm.2022.07.010 Issa, S. S., Shaimardanova, ... Cell, 184(19), 4919-4938.e22. https://doi.org/10.1016/j.cell.2021.08.028 Tervo, D. G. R., Hwang, B.-Y...

... Antibodies 101: Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our most...our CRISPR 101 eBook and the upcoming release of the second edition of our Viral Vectors 101 eBook. We’...factor library, in lentiviral vectors. At 3500 plasmids, this represents the largest single deposit in...to announce that we are now able to ship rabies plasmids in our repository to international requestors....

... (more on when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken...Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional resources on the...

...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent... ligands by exponential enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Read the paper in Science Find the new SHERLOCK plasmids More precision with dual-nuclease Cas9-Cas9 ...Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants...post Read the paper in Science Find the Cas14 plasmids Anti-CRISPR proteins for optogenetic control...paper in Nature Methods Find the anti-CRISPR plasmids Tagging human transcription factors with GFP...

...also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry ...resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid from bacteria...resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. The following...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...

... New to viral vectors? Browse our Viral Vectors 101 blog posts Read blog posts about neuroscience Resources...viral preps for your research Find chemogenetics plasmids for your research ...Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Saloman, J. L., Scheff, N....

....  Table 1: Monomeric FP Timers and Associated Plasmids from Subach et al. Protein Excitation (nm) ...) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red...

...experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment...Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W., Shah, S. S...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...paper in Nature Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase...wheat. Read the paper in Genome Biology Find plasmids containing these base editors Spatially and ...Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA cloning (STAgR) String ...the paper in PLoS One Find the STAgR cloning plasmids Mobile-CRISPRi allows CRISPRi experiments in...

...Western Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...