...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ... al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023). Efficient engineering...inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold...under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C., Goeden...(2023) under CC-BY license.   Find AAV1-X1 plasmid here! Chen, X., et al. (2023). Functional gene ...

...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 156,671 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids from a depositor organized... EXtracellular Plasmid RESource (EXPRESs) Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE...

...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable strain. For plasmids containing...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency... share ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral...brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for intracerebral...wider diffusion is required. On the other hand, lentiviral vectors are superior packaging vectors for larger...astrocytes, and AAV4 targets mostly astrocytes. Lentiviral vectors are pseudotyped in most cases with VSV-G...injection. Find more details about choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector...1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious units per...

... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...

... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...

...on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References...Kantor, B. (2021). Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State... the lentiviruses (like HIV-1) — that have been developed into lentiviral vectors. Lentiviruses are sometimes... 1: Gamma-retrovirus genome (left) and an HIV lentiviral genome (right.)  HIV seems an unlikely candidate...Addgenie Rebecca Hutcheson for her assistance with lentiviral replication.  Resources and References More ...

...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources...

...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...miRNAs to restrict CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision...modification using CRISPR Browse Plasmids: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S....

... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian..., including the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...conducting genome-wide screens using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer...lipids Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...

...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries.... With others, notably pooled lentiviral plasmid libraries, the plasmids must first be used to make virus... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To: Pooled Library...are single preparations of many different plasmids. Plasmids within a given library have the same backbone...libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique...infected cell receives only one plasmid. To make sure that every plasmid is adequately represented in the... given plasmid, think of each cell carrying it as being a biological replicate for that plasmid. Library...

...related to our plasmid collection. Find links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...are single preparations of many different plasmids. Plasmids within a given library have the same backbone...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...

... AAV replication. The transfer plasmid, Rep/Cap, and the helper plasmid are transfected into HEK293 cells...information on optogenetics plasmids available from Addgene. The popular CRISPR/Cas9 genome editing system...Resources AAV Plasmids Available at Addgene Biosafety Guide Parts of an AAV Transfer Plasmid Video Web References...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...capsid proteins). When constructing an AAV transfer plasmid, the transgene is placed between the two ITRs, ...addition to Rep and Cap, AAV requires a helper plasmid containing genes from adenovirus. These genes (...helper genes may also be combined into a single plasmid; the separation of Rep and Cap shown in the figure...

...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains ...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description Origin... more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the...biology basics and plasmid components. Plasmids 101: Origin of Replication Plasmids 101: The Promoter ...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...

...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...

...Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...rapidly initiate a secondary immune response. Browse Plasmids for Immunology Research Genes have been grouped...