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  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...-mutagenesis also requires making your own electrocompetent cells in which the phage recombination machinery...Purified Enzymes Required No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...(anthocyanins), and the plant circadian clock. Petunias have been a model organism for plant research ...mechanisms and components of the circadian rhythm in petunias. Because of the extensive research that has come...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    ...particularly enriched in highly transcribed regions and repetitive regions, such as telomeres and centromeres. These...presumably due to their abundance of R-loops and repetitive sequence. All of the R-loops described above...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...into the host genome, the virus is replication incompetent, meaning it can’t replicate and continue the ...Viral cargo-specific safety   After replication competency, the second concern with virus safety is the ...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather decant ...material that is intended for reuse (e.g. Pasteur pipettes for liquid waste) should be sterilized before ...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...division. These non-plasmid-containing bacteria can compete with and confound the goal of your experiment —...express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy...

  13. An Introduction to Adenovirus

    Type
    Blog Post
    ...virions) are not expressed. Note that replication-competent AdV vectors also exist and are used in certain...topic, which is that natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...system, the possibility of creating a replication-competent virus via recombination between the transfer and...recombination events necessary to create a replication-competent virus. Second, Tat is eliminated through the addition...

  15. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...single colony with a sterile flat toothpick or pipette tip and swirl in a small amount of sterile water...loading dye with glycerol to your samples before pipetting them onto the gel. The figure above summarizes...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  20. March for Science

    Type
    Blog Post
    ...References March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student... 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns Over Politicization...
Showing: 101 - 120 of 292 results